A MICROTITER PLATE-BASED HIGH-THROUGHPUT DNA PURIFICATION METHOD

被引：18

作者：

WANG, K

GAN, L

BOYSEN, C

HOOD, L

机构：

[1] Department of Molecular Biotechnology, University of Washington, Seattle, WA 98195

来源：

ANALYTICAL BIOCHEMISTRY | 1995年 / 226卷 / 01期

关键词：

D O I：

10.1006/abio.1995.1195

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

A fast, reliable, inexpensive, and high-throughput method to purify DNA has been developed. It is based on DNA amplification by polymerase chain reaction utilizing a mini spin-column made from a 96-well membrane-bottomed assay plate. With this method, 50% of the DNA is recovered routinely using Sephacryl-500HR as filtration media. Purified DNA can then be used in various enzymatic manipulations, such as sequencing and hybridization. This provides an economical alternative protocol for routine large-scale purification of DNA templates for sequencing or other enzymatic manipulations. (C) 1995 Academic Press, Inc.

引用

页码：85 / 90

页数：6

共 16 条

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