A SENSITIVE METHOD FOR DETERMINATION OF PROTEOLYTIC ACTIVITY IN VITRO BY IMMUNOCHEMICAL QUANTITATION OF FIBRINOGEN FRAGMENTS

被引：14

作者：

OHLSSON, K

机构：

[1] Department of Clinical Chemistry, Malmö General Hospital

来源：

CLINICA CHIMICA ACTA | 1969年 / 26卷 / 01期

关键词：

D O I：

10.1016/0009-8981(69)90297-6

中图分类号：

R446 [实验室诊断]; R-33 [实验医学、医学实验];

学科分类号：

1001 ;

摘要：

A method is described that allows investigation of proteolytic enzymes with very low activity or in low concentration. Fibrinogen void of plasminogen is used as substrate. Split products released by the enzymes are demonstrated immunochemically and the amount of split products formed within a certain time is a measure of the proteolytic activity. The lower limit for trypsin is less than 1 nanogram trypsin. © 1969.

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页码：131 / &

相关论文

共 12 条

[1] THE FIBRIN PLATE METHOD FOR ESTIMATING FIBRINOLYTIC ACTIVITY [J].

ASTRUP, T ;

MULLERTZ, S .

ARCHIVES OF BIOCHEMISTRY AND BIOPHYSICS, 1952, 40 (02) :346-351

[2] PLASMINOGEN ASSAY BY MEANS OF LYSIS TIME METHOD [J].

BERG, W ;

KORSANBE.K ;

YGGE, J .

THROMBOSIS ET DIATHESIS HAEMORRHAGICA, 1965, 14 (1-2) :127-&

[3]

BERG W, 1967, ARCH MED SCAND S, P478

[4]

BLOMBACK B, 1956, ARK KEMI, V10, P415

[5] PREPARATION AND PROPERTIES OF 2 NEW CHROMOGENIC SUBSTRATES OF TRYPSIN [J].

ERLANGER, BF ;

COHEN, W ;

KOKOWSKY, N .

ARCHIVES OF BIOCHEMISTRY AND BIOPHYSICS, 1961, 95 (02) :271-&

[6] Isolation from beef pancreas of crystalline trypsinogen, trypsin, a trypsin inhibitor, and an inhibitor-trypsin compound [J].

Kunitz, M ;

Northrop, JH .

JOURNAL OF GENERAL PHYSIOLOGY, 1936, 19 (06) :991-1007

[7] CRYSTALLINE SOYBEAN TRYPSIN INHIBITOR .2. GENERAL PROPERTIES [J].

KUNITZ, M .

JOURNAL OF GENERAL PHYSIOLOGY, 1947, 30 (04) :291-310

[8]

LASSEN M, 1952, ACTA PHYSIOL SCAND, V27, P371

[9] QUANTITATIVE ESTIMATION OF PROTEINS BY ELECTROPHORESIS IN AGAROSE GEL CONTAINING ANTIBODIES [J].

LAURELL, CB .

ANALYTICAL BIOCHEMISTRY, 1966, 15 (01) :45-&

[10] SPLIT PRODUCTS OF FIBRINOGEN AFTER PROLONGED INTERACTION WITH PLASMIN [J].

NILEHN, JE .

THROMBOSIS ET DIATHESIS HAEMORRHAGICA, 1967, 18 (1-2) :89-&