TRANSCORTIN IN RAT-KIDNEY - SUBCELLULAR-DISTRIBUTION OF TRANSCORTIN-SYNTHESIZING POLYRIBOSOMES

被引：7

作者：

KRAUJELIS, K

ULINSKAITE, A

MEILUS, V

机构：

[1] Laboratory for Study of Biologically Active Substances, Institute of Biochemistry, Academy of Sciences of Lithuania, 232021 Vilnius

来源：

JOURNAL OF STEROID BIOCHEMISTRY AND MOLECULAR BIOLOGY | 1991年 / 38卷 / 01期

关键词：

D O I：

10.1016/0960-0760(91)90399-P

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The [H-3]corticosterone-transcortin complexes from kidney cytosol show elution positions of DEAE-cellulose identical to serum transcortin. The incorporation of C-14-labeled amino acids into anti-transcortin-precipitable material of kidney slices has been measured and compared with that of serum transcortin. It was established that kidney synthesized transcortin with an apparent molecular weight of 66 kDa on SDS-electrophoresis which resembles serum corticosteroid-binding globulin. Studies on the binding of [I-125]anti-transcortin-IgG to membrane-bound rat kidney polyribosomes revealed an association of [I-125]anti-transcortin-IgG with a discrete polyribosome fraction in the heavy polyribosome region; free polyribosomes were devoid of antigenic material able to bind antibodies to transcortin.

引用

页码：43 / 47

页数：5

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