RAPID METHOD FOR PROCESSING SOIL SAMPLES FOR POLYMERASE CHAIN-REACTION AMPLIFICATION OF SPECIFIC GENE-SEQUENCES

被引：89

作者：

PILLAI, SD ^{[1
]}

JOSEPHSON, KL ^{[1
]}

BAILEY, RL ^{[1
]}

GERBA, CP ^{[1
]}

PEPPER, IL ^{[1
]}

机构：

[1] UNIV ARIZONA,DEPT SOIL & WATER SCI,TUCSON,AZ 85721

来源：

APPLIED AND ENVIRONMENTAL MICROBIOLOGY | 1991年 / 57卷 / 08期

关键词：

D O I：

10.1128/AEM.57.8.2283-2286.1991

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

Bacterial cells can be differentially separated from soil colloids on the basis of their buoyant densities. By using this principle, a modified sucrose gradient centrifugation protocol has been developed for separating bacterial cells from most of the soil colloids. Since the bacterial cell suspension still contained some colloidal soil particles, which inhibited polymerase chain reaction amplification, a new "double" polymerase chain reaction method of analysis was adopted for amplification of Tn5-specific gene sequences. This new protocol allowed rapid detection of small numbers (1 to 10 CFU/g) of bacterial cells present in soil samples.

引用

页码：2283 / 2286

页数：4

共 16 条

[1]

Ausubel FM., 1995, MOL REPROD DEV, V3rd edn, DOI DOI 10.1002/MRD.1080010210

[2] DETECTION OF COLIFORM BACTERIA IN WATER BY POLYMERASE CHAIN-REACTION AND GENE PROBES [J].