INVOLVEMENT OF NERVE GROWTH-FACTOR AND ITS RECEPTOR IN THE REGULATION OF THE CHOLINERGIC FUNCTION IN AGED RATS

被引：56

作者：

ALBERCH, J ^{[1
]}

PEREZNAVARRO, E ^{[1
]}

ARENAS, E ^{[1
]}

MARSAL, J ^{[1
]}

机构：

[1] UNIV BARCELONA, HOSP BELLVITGE, FAC MED, DEPT BIOL CELLULAR & ANAT PATOL, E-08036 BARCELONA, SPAIN

来源：

JOURNAL OF NEUROCHEMISTRY | 1991年 / 57卷 / 05期

关键词：

NERVE GROWTH FACTOR; NERVE GROWTH FACTOR RECEPTOR; CHOLINE ACETYLTRANSFERASE; AGED RATS;

D O I：

10.1111/j.1471-4159.1991.tb06342.x

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 [生物化学与分子生物学]; 081704 [应用化学];

摘要：

The role of nerve growth factor (NGF) and its receptor (NGFR) in the regulation of cholinergic activity has been studied during the aging process. NGFRs were quantified in cortical membranes using a radioactive binding assay. NGF levels and choline acetyltransferase (ChAT) activity were determined in cortex, hippocampus, neostriatum, and septum. These assays were performed in both adult (6-month-old) and aged (36-month-old) rats. High- and low-affinity I-125-NGF binding sites were present in cortex of adult and aged rats. Furthermore, we observed a decrease in number and affinity of both NGFRs in aged rats. ChAT activity in these rats was lower (approximately 30%) than in adult rats in all the brain regions examined. NGF levels were not modified in cortex and hippocampus and were decreased in neostriatum (55%) and septum (35%). In conclusion, our results suggest that, during the aging process, the cholinergic impairment is related to a decrease in NGF levels in neostriatum but not in cortex and hippocampus. The reduction in level of NGF protein in septum could be due to a decrease in number of high-affinity I-125-NGF binding sites.

引用

页码：1483 / 1487

页数：5

共 44 条

[1]

ALBERCH J, 1991, BRAIN RES, V542, P233

[2]

BASIC FIBROBLAST GROWTH-FACTOR PREVENTS DEATH OF LESIONED CHOLINERGIC NEURONS INVIVO [J].