LACK OF PRODUCTION OF (P)PPGPP IN HALOBACTERIUM-VOLCANII UNDER CONDITIONS THAT ARE EFFECTIVE IN THE EUBACTERIA

被引:12
作者
SCOARUGHI, GL [1 ]
CIMMINO, C [1 ]
DONINI, P [1 ]
机构
[1] UNIV ROMA LA SAPIENZA, DIPARTIMENTO BIOL CELLULARE & SVILUPPO, ROME, ITALY
关键词
D O I
10.1128/jb.177.1.82-85.1995
中图分类号
Q93 [微生物学];
学科分类号
071005 ; 100705 ;
摘要
The stringent halobacterial strain Haloferax volcanii was subjected to a set of physiological conditions different from amino acid starvation that are known to cause production of guanosine polyphosphates [(p)pp Gpp] in eubacteria via the relA-independent (spoT) pathway. The conditions used were temperature upshift, treatment with cyanide, and total starvation, Under none of these conditions were detectable levels of (p)ppGpp observed, This result, in conjunction with our previous finding that (p)ppGpp synthesis does not occur under amino acid starvation, leads to the conclusion that in halobacteria both growth rate control and stringency are probably governed by mechanisms that operate in the absence of ppGpp. During exponential growth, a low level of phosphorylated compounds with electrophoretic mobilities similar, but not identical, to that of (p)ppGpp were observed, The intracellular concentration of these compounds increased considerably during the stationary phase of grow th and with all of the treatments used, The compounds were identified as short-chain polyphosphates identical to those found under similar conditions in Saccharomyces cerevisiae.
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页码:82 / 85
页数:4
相关论文
共 21 条
[1]   STUDIES OF THE GTPASE DOMAIN OF ARCHAEBACTERIAL RIBOSOMES [J].
BEAUCLERK, AAD ;
HUMMEL, H ;
HOLMES, DJ ;
BOCK, A ;
CUNDLIFFE, E .
EUROPEAN JOURNAL OF BIOCHEMISTRY, 1985, 151 (02) :245-255
[2]   PAPER CHROMATOGRAPHY OF PHOSPHORIC ESTERS [J].
BURROWS, S ;
GRYLLS, FSM ;
HARRISON, JS .
NATURE, 1952, 170 (4332) :800-801
[3]   AN IMPROVED METHOD FOR THIN-LAYER CHROMATOGRAPHY OF NUCLEOTIDE MIXTURES CONTAINING 32P-LABELED ORTHOPHOSPHATE [J].
CASHEL, M ;
LAZZARINI, RA ;
KALBACHER, B .
JOURNAL OF CHROMATOGRAPHY, 1969, 40 (01) :103-+
[4]  
Cashel M., 1987, CELL MOL BIOL, P1410
[5]   2 COMPOUNDS IMPLICATED IN FUNCTION OF RC GENE OF ESCHERICHIA COLI [J].
CHASHEL, M ;
GALLANT, J .
NATURE, 1969, 221 (5183) :838-&
[6]   STRINGENCY AND RELAXATION AMONG THE HALOBACTERIA [J].
CIMMINO, C ;
SCOARUGHI, GL ;
DONINI, P .
JOURNAL OF BACTERIOLOGY, 1993, 175 (20) :6659-6662
[7]  
EDLIN G, 1971, J BIOL CHEM, V246, P4371
[8]   GUANOSINE 3'-DIPHOSPHATE 5'-DIPHOSPHATE IS NOT REQUIRED FOR GROWTH RATE-DEPENDENT CONTROL OF RIBOSOMAL-RNA SYNTHESIS IN ESCHERICHIA-COLI [J].
GAAL, T ;
GOURSE, RL .
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1990, 87 (14) :5533-5537
[9]   ANOMALOUS SYNTHESIS OF PPGPP IN GROWING CELLS [J].
GALLANT, J ;
PALMER, L ;
PAO, CC .
CELL, 1977, 11 (01) :181-185
[10]   CHROMATOGRAPHIC-SEPARATION AND IDENTIFICATION OF SHORT-CHAIN ACID-SOLUBLE POLYPHOSPHATES FROM SACCHAROMYCES-CEREVISIAE [J].
GUERRINI, AM ;
BARNI, N ;
DONINI, P .
JOURNAL OF CHROMATOGRAPHY, 1980, 189 (03) :440-444