FUSED CELLS ARE SUITED FOR MICRO-INJECTION

被引：10

作者：

GRAESSMANN, A

GRAESSMANN, M

MUELLER, C

机构：

[1] Institut fuer Molekularbiologie, Biochemie der Freien Universitaet Berlin, D 1000 Berlin 33

来源：

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 1979年 / 88卷 / 02期

关键词：

D O I：

10.1016/0006-291X(79)92066-7

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Volumes transferred per cell by microinjection are enhanced by a factor of 104 to 105 when multinucleated HeLa cells fused by polyethylene glycol 1000 are used as recipients instead of single HeLa cells. Fused HeLa cells are viable and support simian virus 40 gene expression upon microinjection of the viral DNA as do the mononucleated parental cells. © 1979.

引用

页码：428 / 432

页数：5

共 14 条

[1] QUANTITATIVE CORRELATION BETWEEN SIMIAN VIRUS-40 T-ANTIGEN SYNTHESIS AND LATE VIRAL GENE-EXPRESSION IN PERMISSIVE AND NONPERMISSIVE CELLS
GRAESSMANN, A
GRAESSMANN, M
GUHL, E
MUELLER, C
[J]. JOURNAL OF CELL BIOLOGY, 1978, 77 (01) : R1 - R8
[2] REGULATORY FUNCTION OF SIMIAN VIRUS-40 DNA-REPLICATION FOR LATE VIRAL GENE-EXPRESSION
GRAESSMANN, A
GRAESSMANN, M
MUELLER, C
[J]. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1977, 74 (11) : 4831 - 4834
[3] GRAESSMANN A, 1979, CURRENT TOPICS MICRO
[4] GRAESSMANN A, 1979, METHODS ENZYMOL
[5] GRAESSMANN A, 1971, H-S Z PHYSIOL CHEM, V352, P527
[6] EARLY SIMIAN-VIRUS-40-SPECIFIC RNA CONTAINS INFORMATION FOR TUMOR ANTIGEN FORMATION AND CHROMATIN REPLICATION
GRAESSMANN, M
GRAESSMANN, A
[J]. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1976, 73 (02) : 366 - 370
[7] GRAESSMANN M, UNPUBLISHED
[8] GRAESSMANN M, 1976, NATURE, V258, P756
[9] SELECTIVE EXTRACTION OF POLYOMA DNA FROM INFECTED MOUSE CELL CULTURES
HIRT, B
[J]. JOURNAL OF MOLECULAR BIOLOGY, 1967, 26 (02) : 365 - &
[10] DIMETHYL-SULFOXIDE ENHANCES POLYETHYLENE GLYCOL-MEDIATED SOMATIC-CELL FUSION
NORWOOD, TH
ZEIGLER, CJ
MARTIN, GM
[J]. SOMATIC CELL GENETICS, 1976, 2 (03): : 263 - 270

← 1 2 →