DNA VARIATION IN TISSUE-CULTURE-DERIVED RICE PLANTS

被引：121

作者：

MULLER, E ^{[1
]}

BROWN, PTH ^{[1
]}

HARTKE, S ^{[1
]}

LORZ, H ^{[1
]}

机构：

[1] MAX PLANCK INST ZUCHTUNGSFORSCH,W-5000 COLOGNE 30,GERMANY

来源：

THEORETICAL AND APPLIED GENETICS | 1990年 / 80卷 / 05期

关键词：

Methylation; RFLP; Rice; Somaclonal variation; Tissue culture;

D O I：

10.1007/BF00224228

中图分类号：

S3 [农学（农艺学）];

学科分类号：

0901 ;

摘要：

Regenerants of rice were examined by RFLP analysis to determine the occurrence and extent of somaclonal variation. DNA polymorphisms were observed both among plants regenerated from different callus cultures as well as among sibling plants derived from a single callus. Regardless of the basal medium, a higher degree of genetic instability was found among plants regenerated from callus cultures maintained for longer incubation periods (67 days) than among those from shorter incubation periods (28 days). Detailed analysis showed that in several regenerants, there was a close correlation among those plants exhibiting DNA rearrangements and those with apparent methylation changes. Such alterations were observed with both structural and housekeeping genes. © 1990 Springer-Verlag.

引用

页码：673 / 679

页数：7

共 15 条

[1] RESTRICTION SITES CONTAINING CPG SHOW A HIGHER FREQUENCY OF POLYMORPHISM IN HUMAN DNA [J].

BARKER, D ;

SCHAFER, M ;

WHITE, R .

CELL, 1984, 36 (01) :131-138

[2] DNA METHYLATION IN PLANTS AND ITS ROLE IN TISSUE-CULTURE [J].

BROWN, PTH .

GENOME, 1989, 31 (02) :717-729

[3] MOLECULAR-BASIS OF BASE SUBSTITUTION HOTSPOTS IN ESCHERICHIA-COLI [J].

COULONDRE, C ;

MILLER, JH ;

FARABAUGH, PJ ;

GILBERT, W .

NATURE, 1978, 274 (5673) :775-780

[4] OCCURRENCE OF 6-METHYLAMINOPURINE IN DEOXYRIBONUCLEIC ACIDS [J].

DUNN, DB ;

SMITH, JD .

BIOCHEMICAL JOURNAL, 1958, 68 :627-636

[5]

EDALLO S, 1981, MAYDICA, V26, P39

[6] SEQUENTIAL OCCURRENCE OF MUTATIONS IN A GROWING RICE CALLUS [J].

FUKUI, K .

THEORETICAL AND APPLIED GENETICS, 1983, 65 (03) :225-230

[7]

HARTKE S, 1989, J GENET BREED, V93, P205

[8] SOMACLONAL VARIATION - A NOVEL SOURCE OF VARIABILITY FROM CELL-CULTURES FOR PLANT IMPROVEMENT [J].

LARKIN, PJ ;

SCOWCROFT, WR .

THEORETICAL AND APPLIED GENETICS, 1981, 60 (04) :197-214

[9] VARIABILITY AMONG PLANTS AND THEIR PROGENY REGENERATED FROM PROTOPLASTS OF SU/SU HETEROZYGOTES OF NICOTIANA-TABACUM [J].

LORZ, H ;

SCOWCROFT, WR .

THEORETICAL AND APPLIED GENETICS, 1983, 66 (01) :67-75

[10] CYTOGENETIC ANALYSIS OF PLANTS REGENERATED FROM OAT (AVENA-SATIVA) TISSUE-CULTURES - HIGH-FREQUENCY OF PARTIAL CHROMOSOME LOSS [J].

MCCOY, TJ ;

PHILLIPS, RL ;

RINES, HW .

CANADIAN JOURNAL OF GENETICS AND CYTOLOGY, 1982, 24 (01) :37-50

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