EFFECTS AND INTERACTIONS OF 17-BETA-ESTRADIOL, T3 AND 1,25(OH)2D3 ON CULTURED OSTEOBLASTS FROM MATURE RATS

被引：25

作者：

EGRISE, D ^{[1
]}

MARTIN, D ^{[1
]}

NEVE, P ^{[1
]}

VERHAS, M ^{[1
]}

SCHOUTENS, A ^{[1
]}

机构：

[1] FREE UNIV BRUSSELS,CLIN UNIV BRUXELLES,B-1050 BRUSSELS,BELGIUM

来源：

BONE AND MINERAL | 1990年 / 11卷 / 03期

关键词：

Alkaline phosphatase; Estrogen; Osteoblast; Osteocalcin; Triiodothyronine; Vitamin D;

D O I：

10.1016/0169-6009(90)90024-A

中图分类号：

R5 [内科学];

学科分类号：

1002 ; 100201 ;

摘要：

Osteoporosis being frequently associated with hyperthyroidism and, mostly after menopause, with deficiency in estrogens, we tried to elucidate the interactions of estrogens and triiodothyronine (T3) with calcitriol by using cultured osteoblast-like cells obtained from mature rat bone. The tested parameters included [3H]thymidine incorporation, evaluation of the alkaline phosphatase activity of the cell layer and osteocalcin production in the culture medium. At physiological concentrations, 17β-estradiol and T3 stimulated alkaline phosphatase activity, did not enhance osteocalcin production and slightly inhibited [3H]thymidine incorporation. At higher concentrations, 17β-estradiol decreased the alkaline phosphatase and osteocalcin response to calcitriol whereas T3 although decreasing alkaline phosphatase activity, markedly increased the osteocalcin secretion elicited by calcitriol. These observations emphasize the complex physiology of osteoblasts and confirm different behaviors of alkalins phosohatase and of osteocalcin as markers of bone turnover. © 1990.

引用

页码：273 / 283

页数：11

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