APPLICATION OF SODIUM DODECYL-SULFATE GEL-ELECTROPHORESIS TO LOW-MOLECULAR-WEIGHT POLYPEPTIDES

Experiments with nine polypeptides with molecular weights between 2000 and 10,760 confirm the value of sodium dodecy sulfate (SDS)-gel electrophoresis for separating polypeptides in this molecular weight range. In one case, electrophoretic biotting and microsequencing were successfully carried out. However, molecular weight determination in the low molecular weight range (<10,000) is much less reliable than that in the conventional molecular weight range (>10,000) for SDS gels. Information provided by suppliers of horse heart myoglobin fragment kits is potentially misleading. © 1990.