PROMOTER PROBE AND SHUTTLE PLASMIDS FOR DEINOCOCCUS-RADIODURANS

被引：28

作者：

MASTERS, CI ^{[1
]}

MINTON, KW ^{[1
]}

机构：

[1] UNIFORMED SERV UNIV HLTH SCI,FF HEBERT SCH MED,DEPT PATHOL,4301 JONES BRIDGE RD,BETHESDA,MD 20814

来源：

PLASMID | 1992年 / 28卷 / 03期

关键词：

D O I：

10.1016/0147-619X(92)90057-H

中图分类号：

Q3 [遗传学];

学科分类号：

071007 ; 090102 ;

摘要：

Two improved Deinococcus radiodurans-Escherichia coli shuttle vectors have been constructed. pI3 is a 16-kb plasmid that confers chloramphenicol resistance in D. radiodurans (CmR, cat) and ampicillin resistance in E. coli (ApR) and contains a multiple cloning site that does not interrupt sequences necessary for replication or drug resistance in either host. pI304 is a promoter-probe plasmid that is similar to pI3, but lacks the D. radiodurans promoting sequence for the cat gene, while retaining sequences necessary for replication. © 1992.

引用

页码：258 / 261

页数：4

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