INTRACELLULAR CA2+ REGULATION IN CD3 STIMULATED JURKAT T-CELLS INVOLVES H+ FLUXES

被引:5
作者
BERTHE, P [1 ]
COUSIN, JL [1 ]
BREITTMAYER, JP [1 ]
机构
[1] FAC MED PASTEUR,INSERM,U210,F-06034 NICE,FRANCE
关键词
CALCIUM FLUX; PH-DEPENDENCE; LYMPHOCYTE-T; ACTIVATION; CD3/TCR;
D O I
10.1016/0898-6568(91)90075-6
中图分类号
Q2 [细胞生物学];
学科分类号
071009 ; 090102 ;
摘要
Triggering the CD3/TCR complex of T lymphocytes induces a rapid rise in cytosolic free calcium followed by a slowly declining plateau. The level of this plateau depends on external pH, the more alkalinized media leading to higher values. Neither a pH-dependent binding of mAb, nor a perturbation of internal pH can account for this effect. In a sodium-free medium, or in the presence of dimethylamiloride Ca2+, elevation is accompanied by an acidification of the cells; both of them depend, to the same extent, on external calcium concentration. TPA inhibits CD3-, but not ionomycin-induced Ca2+ and H+ raises, indicating that it acts more probably on Ca2+ influx, rather than on its efflux. These results suggest that intracellular calcium could be regulated by a Ca2+/H+ ATPase which drives H+ in and Ca2+ out. In the presence of external Na+, H+ should return to the medium by the Na+/H+ exchanger.
引用
收藏
页码:453 / 459
页数:7
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