MOLECULAR-GEOMETRY OF ANTIGEN-BINDING BY A MONOCLONAL-ANTIBODY AGAINST 5-METHYLCYTIDINE

被引：35

作者：

PODESTA, A

CASTIGLIONE, MR

AVANZI, S

MONTAGNOLI, G

机构：

[1] UNIV PISA,DEPT ANAT PHYSIOL & ANIM PROD SCI,2 VIALE PIAGGE,I-56124 PISA,ITALY

[2] NATL COUNCIL RES,INST BIOPHYS,I-56127 PISA,ITALY

[3] UNIV PISA,DEPT BOTAN SCI,I-56100 PISA,ITALY

来源：

INTERNATIONAL JOURNAL OF BIOCHEMISTRY | 1993年 / 25卷 / 06期

关键词：

D O I：

10.1016/0020-711X(93)90249-E

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

1. The specificity of a monoclonal IgG1 raised against a 5-methylcytidine-keyhole limpet hemocyanin conjugate was investigated by inhibition experiments with soluble competing antigens. 2. A competitive enzyme immunoassay has been set up, with the antigen immobilized on polystyrene microtitration wells. 3. The analysis of the cross-reaction profile allowed the topography of the antigen antibody interaction to be described. 4. The binding properties of the monoclonal antibody are discussed in terms of both analytical applications and working limitations in the immunochemical study of gene methylation.

引用

页码：929 / 933

页数：5

共 28 条

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