CHANGES IN CARNITINE AND ACETYLCARNITINE IN HUMAN SEMEN DURING CRYOPRESERVATION

被引：13

作者：

GRIZARD, G

LOMBARDVIGNON, N

BOUCHER, D

机构：

[1] Service de Biologie du Développement et de la Reproduction, 63003 Flermont-Ferrand, CHRU 30, Place Henri Dunant

来源：

HUMAN REPRODUCTION | 1992年 / 7卷 / 09期

关键词：

ACETYLCARNITINE; CARNITINE; HUMAN; SEMINAL PLASMA; SPERMATOZOA;

D O I：

10.1093/oxfordjournals.humrep.a137835

中图分类号：

R71 [妇产科学];

学科分类号：

100211 ;

摘要：

L-Carnitine and acetylcarnitine concentrations were determined in spermatozoa and seminal plasma from 15 men, in both fresh ejaculate and frozen-thawed semen with cryoprotective medium. Sperm motility was also evaluated. In fresh samples, the levels of carnitine and acetylcarnitine in seminal plasma were comparable whereas in spermatozoa, acetylcarnitine predominated. Cryopreservation did not change the carnitine and acetylcarnitine levels in seminal plasma nor the carnitine concentration in spermatozoa; by contrast, the acetylcarnitine level in spermatozoa was decreased in 14 cases (110 +/- 8 versus 210 +/- 20 nmol/10(8) cells). This decrease in acetylcarnitine content was greater during semen dilution in cryoprotectant than after the freezing/thawing process. Motility was also decreased in all cases after the freezing/thawing process. These results suggest that acetylcarnitine recovery in spermatozoa is further evidence of the deleterious effect of the cryoprotective medium in the cryopreservation of semen.

引用

页码：1245 / 1248

页数：4

共 28 条

[1]

Arduini A., Rossi M., Mancinelli G., Belfiglio M., Scurti R., Radatn' G.L., Snohet S.B., Effect of L-camitine and acetyl L-carnitine on the human erythrocyte membrane stability and deformability, Life Sci, 47, pp. 2395-2400, (1990)

[2]

Bartellini M., Canale D., Izzo P.L., Giorgi P.M., Meschini P., Menchini-Fabris G.F., L-camitine and acetylcarnitine in human sperm with normal and reduced motility. Ada, Eur. Fertil, 18, pp. 29-31, (1987)

[3]

Barthelemy Royere C.D., Hammahah S., Lebos C., Tharanne M.J., Lansac J., Ultrastructural changes in membranes and acrosome of human sperm during cryopreservation, Arch. Androl, 25, pp. 29-40, (1990)

[4]

Berhman S.J., Ackerman D.R., Freeze preservation of human semen, Am. J. Obstet. Gynecoi, 103, pp. 654-658, (1969)

[5]

Brooks D.E., Carnitine, acetylcamitine and the activity of carnitine acyltransferases in seminal plasma and spermatozoa of men, rams and rats, J. Reprod. Fertil, 56, pp. 667-673, (1979)

[6]

Brotherton J., Cryopreservation of human semen. Arch, Androl, 25, pp. 181-195, (1990)

[7]

Bruns K.A., Casillas E.R., The metabolism of acetylcamitine and acetate by bovine and hamster epididymal spermatozoa, Biol. Reprod, 41, pp. 218-226, (1989)

[8]

Casillas E.R., Accumulation of camitine by bovine spermatozoa during maturation in the epididymis, J. Biol. Oiem, 240, pp. 8227-8232, (1973)

[9]

Casillas E.R., Erickson B.J., The role of camitine in spermatozoan metabolism: Substrate-induced elevations in the acetylation state of camitine and coenzyme A in bovine and monkey spermatozoa, Biol. Reprod, 12, pp. 275-283, (1975)

[10]

Casillas E.R., Villalobos P., Gonzales R., Distribution of camitine and acylcarnitine in the hamster epididymis and in epididymal spermatozoa during maturation, J. Reprod. Fertil, 72, pp. 197-201, (1984)

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