INDUCTION OF INTERLEUKIN-1-BETA MESSENGER-RNA AFTER FOCAL CEREBRAL-ISCHEMIA IN THE RAT

被引：211

作者：

BUTTINI, M ^{[1
]}

SAUTER, A ^{[1
]}

BODDEKE, HWGM ^{[1
]}

机构：

[1] SANDOZ LTD, PRECLIN RES 360-605, CH-4002 BASEL, SWITZERLAND

来源：

MOLECULAR BRAIN RESEARCH | 1994年 / 23卷 / 1-2期

关键词：

INTERLEUKIN-1-BETA; MIDDLE CEREBRAL ARTERY OCCLUSION; BRAIN ISCHEMIA; MESSENGER RNA; IN-SITU HYBRIDIZATION;

D O I：

10.1016/0169-328X(94)90218-6

中图分类号：

Q189 [神经科学];

学科分类号：

071006 ;

摘要：

The expression of interleukin-1beta (IL-1beta) mRNA in the brain in response to cerebral ischaemia in rats was examined using in situ hybridization histochemistry. Focal cerebral ischaemia was induced in spontaneously hypertensive rats by permanent occlusion of the left middle cerebral artery (MCAO). Whereas no IL-1beta mRNA could be detected in non-operated and sham-operated rats, middle cerebral artery occlusion induced the expression of IL-1beta mRNA within 15 min in the ischaemic brain regions prone to become necrotic after 1-2 days. The message appeared as spot-like signals, reached a peak after 3 h and then declined to undetectable levels within 4 days. Additionally, a pronounced but brief induction of IL-1beta mRNA could be detected 1 h after MCAO in the meninges near the watershed zone. The results demonstrate that the inflammatory cytokine IL-1beta is induced in a time-dependent way after brain ischaemia.

引用

页码：126 / 134

页数：9

共 43 条

[1] BRAIN INTERLEUKIN-1 GENE-EXPRESSION INDUCED BY PERIPHERAL LIPOPOLYSACCHARIDE ADMINISTRATION [J].

BAN, E ;

HAOUR, F ;

LENSTRA, R .

CYTOKINE, 1992, 4 (01) :48-54

[2] CORTICOTROPIN-RELEASING FACTOR PRODUCING NEURONS IN THE RAT ACTIVATED BY INTERLEUKIN-1 [J].

BERKENBOSCH, F ;

VANOERS, J ;

DELREY, A ;

TILDERS, F ;

BESEDOVSKY, H .

SCIENCE, 1987, 238 (4826) :524-526

[3] INTERLEUKIN-1 IMMUNOREACTIVE INNERVATION OF THE HUMAN HYPOTHALAMUS [J].

BREDER, CD ;

DINARELLO, CA ;

SAPER, CB .

SCIENCE, 1988, 240 (4850) :321-324

[4]

CHOMCZYNSKI P, 1987, ANAL BIOCHEM, V162, P156, DOI 10.1016/0003-2697(87)90021-2

[5]

DINARELLO CA, 1991, BLOOD, V77, P1627

[6] INTERLEUKIN-1 STIMULATES THE BETA-AMYLOID PRECURSOR PROTEIN PROMOTER [J].

DONNELLY, RJ ;

FRIEDHOFF, AJ ;

BEER, B ;

BLUME, AJ ;

VITEK, MP .

CELLULAR AND MOLECULAR NEUROBIOLOGY, 1990, 10 (04) :485-495

[7]

FONTANA A, 1984, J IMMUNOL, V132, P1837

[8] INTERLEUKIN-1 OF THE CENTRAL-NERVOUS-SYSTEM IS PRODUCED BY AMEBOID MICROGLIA [J].

GIULIAN, D ;

BAKER, TJ ;

SHIH, LCN ;

LACHMAN, LB .

JOURNAL OF EXPERIMENTAL MEDICINE, 1986, 164 (02) :594-604

[9] AMEBOID MICROGLIA AS EFFECTORS OF INFLAMMATION IN THE CENTRAL-NERVOUS-SYSTEM [J].

GIULIAN, D .

JOURNAL OF NEUROSCIENCE RESEARCH, 1987, 18 (01) :155-&

[10] INTERLEUKIN-1 STIMULATION OF ASTROGLIAL PROLIFERATION AFTER BRAIN INJURY [J].

GIULIAN, D ;

LACHMAN, LB .

SCIENCE, 1985, 228 (4698) :497-499

← 1 2 3 4 5 →