REPLICATION-DEFECTIVE ADENOVIRUS TYPE-5 AS AN IN-VITRO AND IN-VIVO GENE-TRANSFER VECTOR IN CHICKENS

被引:8
作者
ADAM, M
OUALIKENE, W
LECOCQ, H
GUITTET, M
ELOIT, M
机构
[1] ECOLE NATL VET,INRA,UNITE GENET MOLEC,F-94704 MAISONS ALFORT,FRANCE
[2] CNEVA,CENT RECH AVICOLE & PORCINE LAB,UNITE PATHOL AVIAIRE,F-22440 PLOUFRAGAN,FRANCE
关键词
D O I
10.1099/0022-1317-76-12-3153
中图分类号
Q81 [生物工程学(生物技术)]; Q93 [微生物学];
学科分类号
071005 ; 0836 ; 090102 ; 100705 ;
摘要
The capacity of E1A gene-deleted and thus replication-defective adenovirus type 5 (Ad5) to transduce foreign genes in chicken embryo fibroblasts (CEF) as well as in chickens was investigated. The lacZ and luciferase genes were successfully transduced in CEF by replication-defective Ad5, demonstrating that these cells possess receptor(s) for binding and penetration of Ad5. A single intramuscular inoculation of Ad-gD, a replication-defective Ad5 harbouring the gD gene of pseudorabies virus, in adult and 1-day-old chickens led to the production of very high titres of specific antibodies. These gD-specific antibodies persisted for at least 56 days. These results demonstrate that replication-defective Ad5, despite its mammalian origin and the deletion of the E1A gene, is a good candidate for developing non-spreading vaccines in poultry.
引用
收藏
页码:3153 / 3157
页数:5
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