RAT-KIDNEY PAPILLA CONTAINS ABUNDANT SYNAPTOBREVIN PROTEIN PARTICIPATES IN THE FUSION OF ANTIDIURETIC HORMONE-REGULATED WATER CHANNEL-CONTAINING ENDOSOMES IN-VITRO

被引:78
作者
JO, IH
HARRIS, HW
AMENDTRADUEGE, AM
MAJEWSKI, RR
HAMMOND, TG
机构
[1] CHILDRENS HOSP,DIV NEPHROL,BOSTON,MA 02115
[2] HARVARD UNIV,SCH MED,DEPT PEDIAT,BOSTON,MA 02115
[3] UNIV WISCONSIN,DEPT MED,MADISON,WI 53792
[4] WILLIAM S MIDDLETON MEM VET AFFAIRS HOSP,MADISON,WI 53792
关键词
D O I
10.1073/pnas.92.6.1876
中图分类号
O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];
学科分类号
07 ; 0710 ; 09 ;
摘要
Antidiuretic hormone (ADH) regulates renal water excretion by altering the permeability of the collecting duct to water. ADH-responsive epithelial cells are the major cell type lining kidney tubules in the inner medulla and papilla. ADH modulates apical membrane water permeability by the insertion and removal of vesicles containing aquaporin collecting duct water channel protein (now termed AQP-2). To identify and characterize proteins responsible for trafficking of AQP-2-containing vesicles, we utilized antibody and cDNA probes to synaptobrevin b (also termed VAMP-2, for vesicle-associated membrane protein 2), a protein that mediates synaptic vesicle exocytosis in the brain and whose structural homologs are now considered to be components of a complex responsible for intracellular vesicle fusion in all cells. We now report that rat kidney inner medulla and papilla contain abundant synaptobrevin protein. Only light endosomes, one of two types of purified papillary AQP-2-containing endosomes, possess synaptobrevin. Light endosomes fuse in vitro by means of an ATP-dependent process that is significantly inhibited when endosomes are preincubated with either anti-synaptobrevin antibody or tetanus toxin. These data define a functional role for a synaptobrevin protein in the fusion of endosomes in vitro. The presence of abundant synaptobrevin proteins in endosomes containing AQP-2 water channels, as well as insulin-sensitive glucose transporters [Cain, C.C., Trimble, W.S. & Lienhard, G.E. (1992) J. Biol. Chem. 267, 11681-11684], and in cells of Malpighian tubules responsible for urine formation in insects [Chin, A.S., Burgess, R.W., Wong, B.R., Schwartz, T.L. & Scheller, R.H. (1993) Gene 131, 175-181] suggests a specialized role for synaptobrevin in vesicle-mediated membrane transport modulated by peptide hormones.
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页码:1876 / 1880
页数:5
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