SPECTROFLUORIMETRIC ASSAY OF VITAMIN B6 ANALOGUES IN BRAIN TISSUE

被引:42
作者
LOO, YH
BADGER, L
机构
[1] The Lilly Research Laboratories, Indianapolis, Indiana
关键词
D O I
10.1111/j.1471-4159.1969.tb06460.x
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Abstract— —A method for the simultaneous assay of the predominant forms of vitamin B6 occurring in brain tissue was developed. Quantitative release of the vitamers was effected by partial removal of lipids with an organic solvent followed by extraction with aqueous metaphosphoric acid. The compounds were separated by ion exchange column chromatography and estimated fluorimetrically. Copyright © 1969, Wiley Blackwell. All rights reserved
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页码:801 / &
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