APPLICATION OF SOLID-PHASE EXTRACTION FOR THE ANALYSIS OF SULFONAMIDES IN MILK BY HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHY

被引：14

作者：

AGARWAL, VK ^{[1
]}

机构：

[1] CONNECTICUT AGR EXPT STN,NEW HAVEN,CT 06504

来源：

JOURNAL OF LIQUID CHROMATOGRAPHY | 1993年 / 16卷 / 17期

关键词：

D O I：

10.1080/10826079308019667

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

A High Performance Liquid Chromatographic (HPLC) method for the analysis of Sulfadiazine, Sulfathiazole, Sulfapyridine, Sulfamerazine, Sulfamethiazole, Sulfamethazine, Sulfachloropyridazme, Sulfadimethoxine, and Sulfaquinoxaline is described. Milk (5 ml) is diluted with 5 ml potassium phosphate buffer (1 M, pH 4.4) and passed through a Cyclobond-I solid phase extraction (SPE) column. Sulfonamides, retained on the SPE column, are eluted with 4 ml methanol. The eluate is further cleaned using an alumina and AGMP-1 ion exchange column to remove coeluting matrix components. The cleaned extract containing sulfonamides is analyzed by HPLC using a reverse phase column and diode array detector at 265 nm.

引用

页码：3793 / 3799

页数：7

共 17 条

[1] MONITORING OF VETERINARY DRUG RESIDUES BY A COMBINATION OF CONTINUOUS-FLOW TECHNIQUES AND COLUMN-SWITCHING HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHY .1. SULFONAMIDES IN EGG, MEAT AND MILK USING POST-COLUMN DERIVATIZATION WITH DIMETHYLAMINOBENZALDEHYDE [J].