ISOLATION AND CHARACTERIZATION OF ZYMOMONAS-MOBILIS MUTANT DEFECTIVE IN PHOSPHATIDYLETHANOLAMINE N-METHYLTRANSFERASE

被引：19

作者：

TAHARA, Y

YAMASHITA, T

SOGABE, A

OGAWA, Y

机构：

[1] Department of Applied Biological Chemistry, Faculty of Agriculture, Shizuoka University, Shizuoka 422

[2] Department of Applied Biological Chemistry, Faculty of Agriculture, Shizuoka University

来源：

JOURNAL OF GENERAL AND APPLIED MICROBIOLOGY | 1994年 / 40卷 / 05期

关键词：

D O I：

10.2323/jgam.40.389

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

Two mutants of Zymomonas mobilis defective in phosphatidylethanolamine N-methyltransferase have been isolated. One mutant was completely defective in all of the three N-methyltransferase activities dependent on phosphatidylethanolamine, phosphatidyl-N-monomethylethanolamine and phosphatidyl-N,N-dimethylethanolamine, respectively. The other was a leaky mutant in which the three enzyme activities were all equally reduced to 3.4-6.7% of those in the parent strain. Two revertants were derived from the leaky mutant, and in both all three enzyme activities were restored to nearly the same levels as those in the wild-type strain.

引用

页码：389 / 396

页数：8

共 23 条

[1]

AMBRON RT, 1976, FORM FUNCTION PHOSPH, P289

[2]

ANSELL GB, 1982, PHOSPHOLIPIDS, P1

[3]

BLIGH EG, 1959, CAN J BIOCH PHYSL, V37, P891

[4]

BRADFORD MM, 1976, ANAL BIOCHEM, V72, P248, DOI 10.1016/0003-2697(76)90527-3