INTERLEUKIN-1-BETA CONVERTING-ENZYME IN MURINE LANGERHANS CELLS AND EPIDERMAL-DERIVED DENDRITIC CELL-LINES

被引：48

作者：

ARIIZUMI, K ^{[1
]}

KITAJIMA, T ^{[1
]}

BERGSTRESSER, PR ^{[1
]}

TAKASHIMA, A ^{[1
]}

机构：

[1] UNIV TEXAS, SW MED CTR, DEPT DERMATOL, DALLAS, TX 75235 USA

来源：

EUROPEAN JOURNAL OF IMMUNOLOGY | 1995年 / 25卷 / 08期

关键词：

LANGERHANS CELLS; INTERLEUKIN-1-BETA CONVERTING ENZYME; INTERLEUKIN-1-BETA;

D O I：

10.1002/eji.1830250803

中图分类号：

R392 [医学免疫学]; Q939.91 [免疫学];

学科分类号：

100102 ;

摘要：

Interleukin (IL)-1 beta plays an essential role in the induction of T cell-mediated immune responses in skin. Langerhans cells (LC), which constitutively express IL-1 beta mRNA, have been assumed to be the primary source of IL-1 beta in murine epidermis. The purpose of this study was to determine whether LC express mRNA for the IL-1 beta converting enzyme (ICE), a protease that is required for processing pro-IL-1 beta into an active form. Here, we report that both IL-1 beta and ICE mRNA are expressed by the Ia(+) population (i.e. LC) in murine epidermis. Moreover, murine epidermal-derived DC Lines (XS series) also express both IL-1 beta and ICE mRNA, and they secrete relatively large amounts of IL-1 beta following lipopolysaccharide (LPS) stimulation. Finally, LPS-triggered IL-1 beta secretion by XS cells is blocked almost completely by the ICE inhibitor acetyl-Tyr-Val-Ala-Asp-CH2OC(O)-[2,6-(CF3)(2)]Ph. These results demonstrate that LC are the primary source of IL-1 beta within the epidermis, and suggest that the proinflammatory role of IL-1 beta may be regulated pharmacologically by ICE inhibitors in vivo.

引用

页码：2137 / 2141

页数：5

共 40 条

[1] Arend W.P., Prog. Growth Factor Res., 2, (1990)
[2] Dinarello C.S., Thompson R.C., Immunol. Today, 12, (1991)
[3] Dinarello C.A., Semin. Immunol., 4, (1992)
[4] Ohlsson K., Bjork P., Bergenfeldt M., Hagman R., Thompson R.C., Nature, 348, (1990)
[5] Smith R.J., Chin J.E., Sam L.M., Justen J.M., Arthritis Rheum., 34, (1991)
[6] Grassi J., Roberge C.J., Frobert Y., Pradelles P., Poubelle P.E., Immunol. Rev., 119, (1991)
[7] Kronheim S.R., Mumma A., Greenstreet T., Glackin P.J., van Ness K., March C.J., Black R.A., Arch. Biochem. Biophys., 296, (1992)
[8] Hazuda D.J., Strickler J., Simon P., Young P.R., J. Biol. Chem., 266, (1991)
[9] Carruth L.M., Demczuk S., Mizel S.B., J. Biol. Chem., 266, (1991)
[10] Thornberry N.A., Bull H.G., Calaycay J.R., Chapman K.T., Howard A.D., Kosturas M.J., Miller D.K., Molineaux S.M., Weidner J.R., Aunins J., Elliston K.O., Ayala J.M., Casanol F.J., Chin J., Ding G.F.-F., Egger L.A., Gaffney E.P., Limjuco G., Palyhat O.C., Raju S.M., Rolando A.M., Salley J.P., Yamin T.-T., Lee T.D., Shively J.E., MacCross M., Mumford R.A., Schmidt J.A., Tocci M.J., Nature, 356, (1992)

← 1 2 3 4 →