BIOSYNTHESIS OF THE TROPANE-RELATED CYANOBACTERIAL TOXIN ANATOXIN-ALPHA - ROLE OF ORNITHINE DECARBOXYLASE

被引：20

作者：

GALLON, JR

CHIT, KN

BROWN, EG

机构：

[1] Department of Biochemistry, University College of Swansea, Swansea, SA2 8PP, Singleton Park

来源：

PHYTOCHEMISTRY | 1990年 / 29卷 / 04期

关键词：

alkaloid; Anabaena flos-aquae; anatoxin-a; biosynthesis; cyanobacteria; ornithine decarboxylase; putrescine; toxin; tropane;

D O I：

10.1016/0031-9422(90)85413-A

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

A study was made of the biosynthesis by Anabaena flos-aquae of the tropane-related alkaloid anatoxin-a. Evidence is presented that the toxin arises from ornithine via putrescine (1,4-diaminobutane) and that ornithine decarboxylase (EC 4.1.1.17) is involved. An ornithine decarboxylase preparation, with optimal activity at pH 8, was obtained from Anabaena flos-aquae and partially purified by gel-filtration chromatography on DEAE-cellulose. One major and one minor peak of enzymic activity were obtained with Km values of 1.25 and 2.5 mM, respectively. Plasmid DNA (10 Kb; Mr 6.5 × 1016) was detected in the toxic strain of Anabaena flos-aquae but not in a non-toxic strain. DNA from the toxin-producing strain of Anabaena flos-aquae transforms the non-toxic into a toxic strain. © 1990.

引用

页码：1107 / 1111

页数：5

共 31 条

[1] ANATOXIN-A INTERACTIONS WITH CHOLINERGIC SYNAPTIC MOLECULES [J].

ARONSTAM, RS ;

WITKOP, B .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA-BIOLOGICAL SCIENCES, 1981, 78 (07) :4639-4643

[2]

BARNETT GR, 1984, J BIOL CHEM, V259, P179

[3] SYNTHESIS OF ANATOXIN-A VIA INTRA-MOLECULAR CYCLIZATION OF IMINIUM SALTS [J].

BATES, HA ;

RAPOPORT, H .

JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 1979, 101 (05) :1259-1265

[4]

BRODA P, 1979, PLASMIDS

[5] SYNTHESIS OF NOR-ANATOXIN-A AND ANATOXIN-A [J].

CAMPBELL, HF ;

EDWARDS, OE ;

KOLT, R .

CANADIAN JOURNAL OF CHEMISTRY-REVUE CANADIENNE DE CHIMIE, 1977, 55 (08) :1372-1379

[6] TOXICOLOGY AND PHARMACOLOGICAL ACTION OF ANABAENA FLOS-AQUAE TOXIN [J].

CARMICHAEL, WW ;

BIGGS, DF ;

GORHAM, PR .

SCIENCE, 1975, 187 (4176) :542-544

[7]

CARMICHAEL WW, 1982, S AFR J SCI, V78, P367

[8]

Codd G. A., 1988, Biochemistry of the Algae and Cyanobacteria . Proceedings of The Phytochemical Society of Europe, No 28., P283

[9] EFFICIENT TOTAL SYNTHESIS OF (+/-)-ANATOXIN-A [J].

DANHEISER, RL ;

MORIN, JM ;

SALASKI, EJ .

JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 1985, 107 (26) :8066-8073

[10] ANATOXIN-A, A TOXIC ALKALOID FROM ANABAENA-FLOS-AQUAE NRC-44H [J].

DEVLIN, JP ;

EDWARDS, OE ;

GORHAM, PR ;

HUNTER, NR ;

PIKE, RK ;

STAVRIC, B .

CANADIAN JOURNAL OF CHEMISTRY-REVUE CANADIENNE DE CHIMIE, 1977, 55 (08) :1367-1371

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