ISOLATION AND CHARACTERIZATION OF MONOCLONAL-ANTIBODIES MONOSPECIFIC FOR BOVINE ALPHA-CASEIN AND BETA-CASEIN

被引：15

作者：

KUZMANOFF, KM

ANDRESEN, J

BEATTIE, CW

机构：

[1] Center for Cancer Research and Education, University of Illinois School of Medicine at Chicago, Chicago, Illinois

来源：

JOURNAL OF DAIRY SCIENCE | 1991年 / 74卷 / 03期

关键词：

MONOCLONAL ANTIBODIES; ALPHA-S-1-CASEIN; BETA-CASEIN;

D O I：

10.3168/jds.S0022-0302(91)78228-3

中图分类号：

S8 [畜牧、动物医学、狩猎、蚕、蜂];

学科分类号：

0905 ;

摘要：

Two monoclonal antibodies monospecific for bovine alpha-s1-casein, which recognize three genetic variants of alpha-s1-casein, have been isolated and their binding properties characterized. Antibodies 57-115 and 57-310 recognize different antigenic determinants on the alpha-s1 protein with affinity constants of 1.63 x 10(11) and 2.13 x 10(11) M-1, respectively. Five monoclonal antibodies, 58-409, 58-416, 58-488, 58-504, and 58-557, monospecific for bovine beta-casein with affinity constants > 10(9) M-1, which recognize a similar epitope(s) also were isolated. All seven antibodies are of isotype IgG1 and recognize both the denatured and undenatured forms of their antigen, making them suitable for qualitative and quantitative radioimmunoassay.

引用

页码：803 / 810

页数：8

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