ELIMINATION OF THE TEMPERATURE-INDUCED LOSS OF THE ENANTIOSELECTIVITY OF CHEMICALLY BONDED ALBUMIN

被引：8

作者：

SIMEK, Z

VESPALEC, R

SUBERT, J

机构：

[1] CZECHOSLOVAK ACAD SCI, INST ANALYT CHEM, KOUNICOVA 42, CS-61142 BRNO, CZECHOSLOVAKIA

[2] INST NATL HLTH, DRUG CONTROL LAB, CS-61400 BRNO, CZECHOSLOVAKIA

来源：

JOURNAL OF CHROMATOGRAPHY | 1991年 / 543卷 / 02期

关键词：

D O I：

10.1016/S0021-9673(01)95799-1

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

The enantioselectivity of bonded bovine serum albumin (BSA) which has been destroyed by high temperatures can be restored by exposure to methanol, common water-based mobile phases are inefficacious. The effectiveness of methanol demonstrates that BSA enantioselectivity, though conformationally dependent, is not conditioned by native conformation of BSA. Therefore, a systematic study is advised of the influence of denaturing factors on BSA enantioselectivity.

引用

页码：475 / 478

页数：4

共 7 条

[1] OPTICAL RESOLUTION BY LIQUID-CHROMATOGRAPHY ON IMMOBILIZED BOVINE SERUM-ALBUMIN [J].