FIBRINOLYTIC BEHAVIOR OF STREPTOKINASE-IMMOBILIZED POLY(METHACRYLIC ACID-G-ETHYLENE OXIDE)

被引：12

作者：

DRUMMOND, RK ^{[1
]}

PEPPAS, NA ^{[1
]}

机构：

[1] PURDUE UNIV, SCH CHEM ENGN, W LAFAYETTE, IN 47907 USA

来源：

BIOMATERIALS | 1991年 / 12卷 / 04期

基金：

美国国家科学基金会;

关键词：

FIBRIN; POLY(METHACRYLIC ACID); POLY(ETHYLENE OXIDE); GRAFTING; COPOLYMERS;

D O I：

10.1016/0142-9612(91)90002-R

中图分类号：

R318 [生物医学工程];

学科分类号：

0831 ;

摘要：

Streptokinase was immobilized on poly(methacrylic acid-g-ethylene oxide) surfaces by means of a modified coupling reagent method. The surfaces were activated with a carbodiimide to form an O-acyl isourea derrivative, which, upon addition of the enzyme, condensed to form the corresponding amide. The quantity of enzyme immobilized on the surface increased as the reaction time with carbodiimide and streptokinase increased. The fibrinolytic activity of the immobilized enzyme systems was measured by a clot lysis assay. The dynamics of fibrin clot lysis was investigated for a period of up to 500 min. The lysis reaction mechanism was found to approximate a first-order fibrin degradation. In addition, the kinetic rate constant was found to increase with increasing immobilized enzyme content.

引用

页码：356 / 360

页数：5

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