A LYSINE SENSOR FOR PROCESS-CONTROL

被引：21

作者：

SIEGLER, K

WEBER, B

WEBER, E

TONDER, K

KLINGNER, E

RIECHERT, F

机构：

[1] UNIV HALLE WITTENBERG,FACHBEREICH MED,INST BIOCHEM,HOLLYSTR 1,D-06097 HALLE,GERMANY

[2] FZB BIOTECHN GMBH BERLIN,D-12489 BERLIN,GERMANY

[3] UNIV HALLE WITTENBERG,FACHBEREICHS BIOL,INST MIKROBIOL,D-06120 HALLE,GERMANY

[4] SITZ FREITAL,PRUFGERATEWERK MEDINGEN,D-01691 FREITAL,GERMANY

来源：

JOURNAL OF CHEMICAL TECHNOLOGY AND BIOTECHNOLOGY | 1994年 / 59卷 / 03期

关键词：

LYSINE FERMENTATION; PROCESS CONTROL; BIOSENSOR; L-LYSINOXIDASE; LYSINE OXIDASE ELECTRODE;

D O I：

10.1002/jctb.280590311

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

A lysine sensor for process control of lysine fermentation was developed based on a Clark-type electrode in combination with L-lysine-alpha-oxidase. The enzyme, isolated from Trichoderma viride, was immobilized between a cellulose and a polypropylene foil using a polyurethane resin. Lysine determinations were carried out in a flow-through system as anodic measurements when H2O2 was measured and as cathodic measurements when the consumption of O2 was followed. The sensitivity of the sensor toward other amino acids was determined.

引用

页码：279 / 287

页数：9

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