SELF-INCOMPATIBILITY IN PETUNIA-INFLATA - ISOLATION AND CHARACTERIZATION OF CDNAS ENCODING 3 S-ALLELE-ASSOCIATED PROTEINS

被引：141

作者：

AI, YJ ^{[1
]}

SINGH, A ^{[1
]}

COLEMAN, CE ^{[1
]}

IOERGER, TR ^{[1
]}

KHEYRPOUR, A ^{[1
]}

KAO, TH ^{[1
]}

机构：

[1] PENN STATE UNIV,DEPT MOLEC & CELL BIOL,UNIVERSITY PK,PA 16802

来源：

SEXUAL PLANT REPRODUCTION | 1990年 / 3卷 / 02期

关键词：

cDNA sequence; Petunia inflata; S-allele-associated proteins; Self-incompatibility;

D O I：

10.1007/BF00198857

中图分类号：

Q94 [植物学];

学科分类号：

071001 ;

摘要：

We have identified three alleles of the S-locus controlling self-incompatibility and their associated pistil proteins in Petunia inflata, a species that displays monofactorial gametophytic self-incompatibility. These S-allele-associated proteins (S-proteins) are pistil specific, and their levels are developmentally regulated. The amino-terminal sequences determined for the three S-proteins are highly conserved and show considerable homology to those of S-proteins from Petunia hybrida, Nicotiana alata and Lycopersicon peruvianum, three other species of the Solanaceae that also exhibit gametophytic self-incompatibility. cDNA clones encoding the three S-proteins were isolated and sequenced. Comparison of their deduced amino acid sequences reveals an average homology of 75.6%, with conserved and variable residue interspersed throughout the protein. Of the 137 conserved residues, 53 are also conserved in the N. alata S-proteins studies so far; of the 64 variable residues, 29 were identified as hypervariable based on calculation of the Similarity Index. There is only one hypervariable region of significant length, and it consists of eight consecutive hypervariable residues. This region correspond approximately to the hypervariable region HV2 identified in N. alata S-proteins. Of the two classes of N. alata S-proteins previously identified, one class exhibits greater homology to the three P. inflata S-proteins reported here than to the other class of N. alata S-proteins. © 1990 Springer-Verlag.

引用

页码：130 / 138

页数：9

共 18 条

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