NONWOVEN FABRICS AS NEW CELL MATRICES FOR IMR-90 HUMAN EMBRYONIC LUNG DIPLOID FIBROBLAST CELLS

IMR-90 cells could proliferate on non-woven fabrics (NWF) up to a cell density of about 3 × 105/cm2, which was about 7 times higher compared to that in monolayer culture with a T-flask. The production of tissue plasminogen activator (t-PA) by perfusion culture using a device which includes a cultivating pan fitted inside a sheet of NWF was performed. The t-PA activity at a dilution rate of 0.22, 0.33 and 0.45 d-1 was 245, 220 and 160 IU/ml. Compared to plastic surfaces, NWF stably retained the cells during t-PA production using serum-free medium. © 1990.