THE ANTIGENIC STRUCTURE OF THE HUMAN GLYCOPROTEIN HORMONE ALPHA-SUBUNIT .2. CROSS-SPECIES COMPARISONS

被引：9

作者：

BERGERT, ER

MADDEN, B

MCCORMICK, DJ

PAPKOFF, H

RYAN, RJ

机构：

[1] MAYO CLIN & MAYO GRAD SCH MED,DEPT BIOCHEM & MOLEC BIOL,ROCHESTER,MN 55901

[2] UNIV CALIF SAN FRANCISCO,HORMONE RES LAB,SAN FRANCISCO,CA 94143

来源：

ENDOCRINOLOGY | 1990年 / 127卷 / 06期

关键词：

D O I：

10.1210/endo-127-6-2985

中图分类号：

R5 [内科学];

学科分类号：

1002 ; 100201 ;

摘要：

Eight monoclonal antibodies, specific for the glycoprotein hormone α-subunit, were raised against human free α-subunit, human FSH, or human CG. All of these antihuman monoclonal antibodies were tested for cross-reactivity with α-subunits derived from bovine, porcine, equine, bull frog, sea turtle, turkey, and ostrich glycoprotein hormones. All showed cross-reactivity with affinities ranging from 10-4to 10-8depending upon the antibody and the species of α-subunit. Cyanogen bromide fragments of bovine and equine α, when tested with selected antibodies indicated that antigenic determinants could be localized in two regions: α9-33 and α76-92. Comparison of amino acid sequences, and relative potencies, suggest that major antigenic determinants involve residues 21, 22, and 23 (F-F-S in human α) and 76-85 (G-G-F-K-V-E-N-H-T-A in human α). As part of this study the N-terminal amino acid sequences of bull frog, sea turtle, turkey, and ostrich α-subunits were determined and reported for the first time. © 1990 by The Endocrine Society.

引用

页码：2985 / 2989

页数：5

共 25 条

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