DOT IMMUNOBINDING ASSAY IN COMPARISON WITH ENZYME-LINKED-IMMUNOSORBENT-ASSAY FOR THE DETECTION OF BLUETONGUE VIRUS-ANTIBODIES IN SHEEP

被引：15

作者：

GUPTA, Y

CHAND, P

SINGH, A

JAIN, NC

机构：

[1] Department of Veterinary Microbiology, College of Veterinary Sciences, Haryana Agricultural University, Hisar

来源：

VETERINARY MICROBIOLOGY | 1990年 / 22卷 / 04期

关键词：

D O I：

10.1016/0378-1135(90)90023-O

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

A total of 384 sheep serum samples collected from two organised sheep farms was tested by dot immunobinding assay (DIA) and indirect enzyme-linked immunosorbent assay (I-ELISA) for the presence of bluetongue virus (BTV) antibodies. The results of both these assays were compared to find a sensitive, specific, rapid, easily performed and economical test for the diagnosis of bluetongue disease. DIA detected BTV antibodies in 210 samples (54.94%) and I-ELISA detected 157 positive samples (40.88%). Competitive ELISA (C-ELISA) was performed to check the discrepancies in I-ELISA and DIA. On the basis of these tests the overall agreement, relative specificity and sensitivity between ELISA and DIA were 75%, 87.6% and 100%, respectively. DIA was found to be a rapid, sensitive, easily performed and economical test as compared to ELISA. © 1990.

引用

页码：365 / 371

页数：7

共 27 条

[1] TEMPORAL DEVELOPMENT OF BLUETONGUE VIRUS PROTEIN-SPECIFIC ANTIBODY IN SHEEP FOLLOWING NATURAL INFECTION [J].