NEW SEPHAROSE DERIVATIVE CONTAINING COVALENTLY BOUND MYOINOSITOL - ITS STRUCTURE AND APPLICATION

被引：4

作者：

FAZLDEEN, H

BREITENBACH, M

机构：

[1] VIENNA UNIV, INST ALLGEMEINE CHEM, A-1090 VIENNA, AUSTRIA

[2] LUDWIG BOLTZMANN FORSCHUNGSSTELLE BIOCHEM, A-1090 Vienna, AUSTRIA

来源：

MONATSHEFTE FUR CHEMIE | 1979年 / 110卷 / 04期

关键词：

Affinity chromatography; Epoxy coupling; Myo-inositol; Sepharose;

D O I：

10.1007/BF00906676

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

myo-Inositol was covalently bound on, an epoxy-activated sepharose. In order to elucidate the structure of the derivative of sepharose formed, in a model reaction myo-inositol was coupled to propylene oxide, whereby two compounds were obtained. By NMR-spectroscopy,13C-resonancespectroscopy and gas chromatography substance I could be characterized as a mixture of two isomers. Both are the product of a binding to the hydroxyl group on C-atom 2 of myo-inositol. The only difference seems to be the point of attachment to the side chain. Substance II, however, originates by substitution on an equitorial hydroxyl group. The application of this new affinity gel is reported. © 1979 Springer-Verlag.

引用

页码：823 / 830

页数：8

共 8 条

[1]

BOLLMANN O, UNPUBLISHED

[2]

HASLINGER E, UNPUBLISHED

[3]

JACKMAN LM, 1969, APPLICATIONS NUCLEAR, V5, P195

[4] SYNTHESIS OF A SPECIFICALLY SUBSTITUTED SEPHAROSE DERIVATIVE FOR AFFINITY CHROMATOGRAPHY OF ENZYMES ACTING ON MYOINOSITOL [J].

KOLLER, F ;

HOFFMANN.O .

MONATSHEFTE FUR CHEMIE, 1974, 105 (02) :379-381

[5]

KOLLER F, HOPPESEYLERS Z PHYSL

[6]

MULLER E, 1965, METHODEN ORGANISCHEN, V6, P42

[7]

PORATH J, 1970, J CHROMATOGR, V51, P479

[8]

SCHEINER O, 1979, MH CHEM, V107, P581

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