ESCHERICHIA-COLI ORFE (UPSTREAM OF PYRE) ENCODES RNASE PH

被引：16

作者：

OST, KA ^{[1
]}

DEUTSCHER, MP ^{[1
]}

机构：

[1] UNIV CONNECTICUT,CTR HLTH,DEPT BIOCHEM,FARMINGTON,CT 06030

来源：

JOURNAL OF BACTERIOLOGY | 1991年 / 173卷 / 17期

关键词：

D O I：

10.1128/jb.173.17.5589-5591.1991

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

RNase PH from extracts of Escherichia coli was purified to homogeneity and subjected to NH2-terminal sequencing. Comparison of this sequence with all open reading frames in the GenBank data base revealed at least 95% identity to an unidentified open reading frame (orfE) upstream of pyrE at 81.7 min on the E. coli chromosome. Clones of orfE overexpress RNase PH activity, verifying that orfE encodes this ribonuclease. We suggest that orfE be renamed rph.

引用

页码：5589 / 5591

页数：3

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