2 PROMOTER ELEMENTS ARE NECESSARY AND SUFFICIENT FOR EXPRESSION OF THE SEA-URCHIN U1 SNRNA GENE

被引：14

作者：

WENDELBURG, BJ ^{[1
]}

MARZLUFF, WF ^{[1
]}

机构：

[1] FLORIDA STATE UNIV,INST MOLEC BIOPHYS,DEPT CHEM,TALLAHASSEE,FL 32306

来源：

NUCLEIC ACIDS RESEARCH | 1992年 / 20卷 / 14期

关键词：

D O I：

10.1093/nar/20.14.3743

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The essential elements of the sea urchin L. variegatus U1 snRNA promoter were mapped by microinjection of a U1 maxigene into sea urchin zygotes. Two elements are required for expression: a distal sequence element (DSE) located between - 318 and - 300 and a proximal sequence element (PSE) centered at - 55. Removal or alteration of other sequences conserved in different sea urchin snRNA U1 genes, including deletion of all sequence between - 90 and - 273, did not affect the expression. Sequences around the start site were not required for expression. Deletion of nucleotides between the PSE and the start site resulted in initiation inside the U1 coding region, suggesting that the PSE determines the start site of transcription. There is no obvious similarity between the sequences required for the sea urchin U1 snRNA expression and the sequences required for the expression of other sea urchin snRNAs.

引用

页码：3743 / 3751

页数：9

共 32 条

[1] DISTINCT FACTORS WITH SP1 AND NF-A SPECIFICITIES BIND TO ADJACENT FUNCTIONAL ELEMENTS OF THE HUMAN U2 SNRNA GENE ENHANCER [J].

ARES, M ;

CHUNG, JS ;

GIGLIO, L ;

WEINER, AM .

GENES & DEVELOPMENT, 1987, 1 (08) :808-817

[2] ORIENTATION-DEPENDENT TRANSCRIPTIONAL ACTIVATOR UPSTREAM OF A HUMAN U2 SNRNA GENE [J].

ARES, M ;

MANGIN, M ;

WEINER, AM .

MOLECULAR AND CELLULAR BIOLOGY, 1985, 5 (07) :1560-1570

[3] A COMMON OCTAMER MOTIF BINDING-PROTEIN IS INVOLVED IN THE TRANSCRIPTION OF U6 SNRNA BY RNA POLYMERASE-III AND U2 SNRNA BY RNA POLYMERASE-II [J].

CARBON, P ;

MURGO, S ;

EBEL, JP ;

KROL, A ;

TEBB, G ;

MATTAJ, IW .

CELL, 1987, 51 (01) :71-79

[4] PROPERTIES OF A U1-RNA ENHANCER-LIKE SEQUENCE [J].

CILIBERTO, G ;

PALLA, F ;

TEBB, G ;

MATTAJ, IW ;

PHILIPSON, L .

NUCLEIC ACIDS RESEARCH, 1987, 15 (06) :2403-2416

[5] CLOSELY LINKED EARLY AND LATE HISTONE H2B GENES ARE DIFFERENTIALLY EXPRESSED AFTER MICROINJECTION INTO SEA-URCHIN ZYGOTES [J].

COLIN, AM ;

CATLIN, TL ;

KIDSON, SH ;

MAXSON, R .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1988, 85 (02) :507-510

[6]

DELORENZI M, 1986, P NATL ACAD SCI USA, V83, P3243

[7] ISOLATION OF AN ACTIVE GENE AND OF 2 PSEUDOGENES FOR MOUSE-U7 SMALL NUCLEAR-RNA [J].

GRUBER, A ;

SOLDATI, D ;

BURRI, M ;

SCHUMPERLI, D .

BIOCHIMICA ET BIOPHYSICA ACTA, 1991, 1088 (01) :151-154

[8] UNIDIRECTIONAL DIGESTION WITH EXONUCLEASE-III CREATES TARGETED BREAKPOINTS FOR DNA SEQUENCING [J].

HENIKOFF, S .

GENE, 1984, 28 (03) :351-359

[9] FUNCTIONAL, DEVELOPMENTALLY EXPRESSED GENES FOR MOUSE U1A AND U1B SNRNAS CONTAIN BOTH CONSERVED AND NONCONSERVED TRANSCRIPTION SIGNALS [J].

HOWARD, EF ;

MICHAEL, SK ;

DAHLBERG, JE ;

LUND, E .

NUCLEIC ACIDS RESEARCH, 1986, 14 (24) :9811-9825

[10] DEVELOPMENTAL AND TISSUE-SPECIFIC EXPRESSION OF U4 SMALL NUCLEAR-RNA GENES [J].

KORF, GM ;

BOTROS, IW ;

STUMPH, WE .

MOLECULAR AND CELLULAR BIOLOGY, 1988, 8 (12) :5566-5569

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