PROCESSING IN THE 5' REGION OF THE PNP TRANSCRIPT FACILITATES THE SITE-SPECIFIC ENDONUCLEOLYTIC CLEAVAGES OF MESSENGER-RNA

被引：9

作者：

TAKATA, R ^{[1
]}

IZUHARA, M ^{[1
]}

AKIYAMA, K ^{[1
]}

机构：

[1] SAGA MED SCH,DEPT BIOL,SAGA 849,JAPAN

来源：

NUCLEIC ACIDS RESEARCH | 1992年 / 20卷 / 04期

关键词：

D O I：

10.1093/nar/20.4.847

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The primary transcript of pnp, the gene encoding polynucleotide phosphorylase in Escherichia coli, is processed in the 5' end region by ribonuclease III (RNase III). The unprocessed transcript shows enhanced stability compared with the processed transcript. We report here that, unlike the processed transcript, the unprocessed pnp transcript did not accept endonucleolytic attack at, at least, five cleavage sites. Sequencing analysis of the four cleavage products shows no sequence specific to all these sites, but AU rich stretches were observed at three sites.

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页码：847 / 850

页数：4

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