DIFFERENTIAL EXPRESSION OF 2 PEANUT PEROXIDASE CDNA CLONES IN PEANUT PLANTS AND CELLS IN SUSPENSION-CULTURE IN RESPONSE TO STRESS

被引：29

作者：

BREDA, C

BUFFARD, D

VANHUYSTEE, RB

ESNAULT, R

机构：

[1] CNRS, INST SCI VEGETALES, F-91198 GIF SUR YVETTE, FRANCE

[2] UNIV WESTERN ONTARIO, DEPT PLANT SCI, LONDON N6A 5B7, ONTARIO, CANADA

来源：

PLANT CELL REPORTS | 1993年 / 12卷 / 05期

关键词：

D O I：

10.1007/BF00237133

中图分类号：

Q94 [植物学];

学科分类号：

071001 ;

摘要：

Peanut (Arachis hypogea L.) peroxidase gene expression was analyzed by measuring the accumulation of trancripts in cultured cells and various plant parts (leaf, stem, root) and upon their treatment with ethylene or wounding, respectively. Two transcripts (prxPNC1 and prxPNC2) corresponding to two peroxidase genes are expressed at higher levels in cultured cells as compared to various plant organs. Analysis of total poly(A)+ RNA with an oligonucleotide probe corresponding to a highly conserved region of peroxidase genes showed the expression of three peroxidase related sequences (1,000, 1,400 or 2,600 bp) in stem or leaf but barely detectable in roots. The prxPNC2 transcript transiently expressed at high levels in response to ethylene treatment of cells or wounding of leaves. This suggests that the corresponding gene(s) are expressed in response to stress.

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页码：268 / 272

页数：5

相关论文

共 23 条

[1] MOLECULAR-CLONING OF COMPLEMENTARY DNAS ENCODING 2 CATIONIC PEROXIDASES FROM CULTIVATED PEANUT CELLS [J].

BUFFARD, D ;

BREDA, C ;

VANHUYSTEE, RB ;

ASEMOTA, O ;

PIERRE, M ;

HA, DBD ;

ESNAULT, R .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1990, 87 (22) :8874-8878

[2] IMMUNOLOGICAL RELATEDNESS OF PEANUT PEROXIDASE FROM CELLS IN SUSPENSION-CULTURES TO PEANUT PLANT [J].

CHIBBAR, RN ;

VANHUYSTEE, RB .

ZEITSCHRIFT FUR PFLANZENPHYSIOLOGIE, 1983, 109 (03) :191-196

[3] THE GROWTH OF AND PEROXIDASE SYNTHESIS BY 2 CARROT CELL-LINES [J].

CHIBBAR, RN ;

CELLA, R ;

ALBANI, D ;

VANHUYSTEE, RB .

JOURNAL OF EXPERIMENTAL BOTANY, 1984, 35 (161) :1846-1852

[4] STRUCTURE OF THE HORSERADISH-PEROXIDASE ISOZYME-C GENES [J].

FUJIYAMA, K ;

TAKEMURA, H ;

SHIBAYAMA, S ;

KOBAYASHI, K ;

CHOI, JK ;

SHINMYO, A ;

TAKANO, M ;

YAMADA, Y ;

OKADA, H .

EUROPEAN JOURNAL OF BIOCHEMISTRY, 1988, 173 (03) :681-687

[5]

GREPPIN H, 1986, MOL PHYSL ASPECTS AS

[6] MESSENGER-RNA FOR G1 PROTEIN OF FRENCH BEAN-SEEDS - CELL-FREE TRANSLATION AND PRODUCT CHARACTERIZATION [J].

HALL, TC ;

BUCHBINDER, BU ;

PYNE, JW ;

SUN, SM ;

BLISS, FA .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1978, 75 (07) :3196-3200

[7] EFFECTS OF CAFFEINE TREATMENT ALONE AND IN COMBINATION WITH GAMMA-EXPOSURE ON CULTURED PEANUT CELLS [J].

INOUE, M ;

VANHUYSTEE, RB .

CANADIAN JOURNAL OF BOTANY-REVUE CANADIENNE DE BOTANIQUE, 1984, 62 (09) :1890-1895

[8] MOLECULAR-CLONING OF COMPLEMENTARY-DNA ENCODING THE LIGNIN-FORMING PEROXIDASE FROM TOBACCO - MOLECULAR ANALYSIS AND TISSUE-SPECIFIC EXPRESSION [J].

LAGRIMINI, LM ;

BURKHART, W ;

MOYER, M ;

ROTHSTEIN, S .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1987, 84 (21) :7542-7546

[9] TISSUE-SPECIFICITY OF TOBACCO PEROXIDASE ISOZYMES AND THEIR INDUCTION BY WOUNDING AND TOBACCO MOSAIC-VIRUS INFECTION [J].

LAGRIMINI, LM ;

ROTHSTEIN, S .

PLANT PHYSIOLOGY, 1987, 84 (02) :438-442

[10] DENOVO SYNTHESIS AND RELEASE OF PEROXIDASES IN CELL-SUSPENSION CULTURES OF NICOTIANA-TABACUM-L [J].

MADER, M ;

WALTER, C .

PLANTA, 1986, 169 (02) :273-277