CRYSTALLIZATION AND PRELIMINARY-X-RAY DIFFRACTION STUDIES OF THEILERS VIRUS (GDVII STRAIN)

Theiler's murine encephalomyelitis virus (TMEV) is a member of the picornavirus family. Mice infected with TMEV serve as models for the study of human neurological diseases including multiple sclerosis. Preparations of the GDVII strain of Theiler's virus have been crystallized using the hanging drop technique. When exposed to high intensity synchrotron radiation, these monoclinic crystals diffracted X-rays to at least 3.0 Å resolution. The unit cell has a C-centered lattice with dimensions: a = 575.2 Å, b = 324.0 Å and c = 558.4 Å, β= 108.2°. The molecular mass and cell dimensions imply that there is an entire virus particle per asymmetric unit, suggesting the presence of 60-fold non-crystallographic redundancy. This GDVII crystal form appears to be suitable for high-resolution structure determination. © 1993 Academic Press, Inc.