EFFECT OF A RECOMBINANT ENDOTOXIN-NEUTRALIZING PROTEIN ON ENDOTOXIN-SHOCK IN RABBITS

被引：26

作者：

GARCIA, C

SALADINO, R

THOMPSON, C

HAMMER, B

PARSONNET, J

WAINWRIGHT, N

NOVITSKY, T

FLEISHER, GR

SIBER, G

机构：

[1] HARVARD UNIV,CHILDRENS HOSP,SCH MED,DEPT MED,DIV EMERGENCY MED,BOSTON,MA 02115

[2] HARVARD UNIV,SCH MED,DANA FARBER CANC INST,INFECT DIS LAB,BOSTON,MA 02115

[3] DARTMOUTH HITCHCOCK MED CTR,DEPT MED,DIV INFECT DIS,LEBANON,NH

[4] ASSOCIATES CAPE COD INC,WOODS HOLE,MA

来源：

CRITICAL CARE MEDICINE | 1994年 / 22卷 / 08期

关键词：

ENDOTOXIN; LIPOPOLYSACCHARIDE; TUMOR NECROSIS FACTOR; SHOCK; SEPSIS; GRAM-NEGATIVE BACTERIA; ESCHERICHIA COLI; INFECTION; ANIMAL MODEL; CRITICAL ILLNESS;

D O I：

10.1097/00003246-199408000-00003

中图分类号：

R4 [临床医学];

学科分类号：

1002 ; 100602 ;

摘要：

Objectives: Limulus anti-lipopolysaccharide factor, an 11.8-kilodalton peptide isolated from amebocytes of Limulus polyphemus inhibits the biologic activities of endotoxin in vitro, including gelation of Limulus amebocyte lysate. A recombinant version of limulus anti-lipopolysaccharide factor, termed endotoxin neutralizing protein, has now been expressed in yeast. Endotoxin-neutralizing protein was evaluated for its potential prophylactic and therapeutic effects in rabbits challenged with Escherichia coli endotoxin. Design: Controlled animal trial. Setting: Animal research laboratory. Subjects: A total of 112 New Zealand white rabbits were studied. Interventions: Rabbits were challenged with an LD80 dose of E. coli endotoxin (100 mu g/kg); control animals (n = 52) were treated with saline solution at the time of endotoxin challenge; experimental animals received endotoxin-neutralizing protein 2.5 mg/kg prechallenge (n 20), 5.0 mg/kg prechallenge (n = 20), or 5.0 mg/kg 30 mins postchallenge (n = 20).

引用

页码：1211 / 1218

页数：8

共 41 条

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