SITE-DIRECTED MUTAGENESIS OF CHITINASE FROM ALTEROMONAS SP STRAIN O-7

被引：33

作者：

TSUJIBO, H ^{[1
]}

ORIKOSHI, H ^{[1
]}

IMADA, C ^{[1
]}

OKAMI, Y ^{[1
]}

MIYAMOTO, K ^{[1
]}

INAMORI, Y ^{[1
]}

机构：

[1] INST MICROBIAL CHEM, SHINAGAWA KU, TOKYO 141, JAPAN

来源：

BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY | 1993年 / 57卷 / 08期

关键词：

D O I：

10.1271/bbb.57.1396

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Chitinase (Chi85) from Alteromonas sp. strain 0-7 contains the two conserved regions common to microbial and plant chitinases. We did site-directed mutagenesis of Chi85 to investigate the effects of the conserved amino acid residues on chitinase activity. We suggest that Asp-290 and Glu-292 of Chi85 may be the essential amino acid residues for the cleavage of beta-glycosidic linkage of chitin.

引用

页码：1396 / 1397

页数：2

共 21 条

[1] PRIMARY STRUCTURE OF A CHITINASE-ENCODING GENE (CHI1) FROM THE FILAMENTOUS FUNGUS APHANOCLADIUM-ALBUM - SIMILARITY TO BACTERIAL CHITINASES [J].