CLONING AND CHARACTERIZATION OF THE RECA GENES FROM MYCOPLASMA-PULMONIS AND M-MYCOIDES SUBSP MYCOIDES

被引：13

作者：

KING, KW

WOODARD, A

DYBVIG, K

机构：

[1] UNIV ALABAMA,DEPT COMPARAT MED,BIRMINGHAM,AL 35294

[2] UNIV ALABAMA,DEPT MICROBIOL,BIRMINGHAM,AL 35294

来源：

GENE | 1994年 / 139卷 / 01期

关键词：

DEGENERATE PRIMERS; INVERSE PCR; PHYLOGENY; ACHOLEPLASMA LAIDLAWII; GRAM(+) BACTERIA;

D O I：

10.1016/0378-1119(94)90532-0

中图分类号：

Q3 [遗传学];

学科分类号：

071007 ; 090102 ;

摘要：

The RecA protein has a central role in DNA repair and is essential for homologous recombination in most eubacteria. Little is known about these critical processes in mycoplasmas. By using standard and inverse polymerase chain reactions (PCR) coupled with conventional cloning techniques, a series of overlapping fragments comprising the entire recA genes of Mycoplasma mycoides subsp. mycoides (Mm) and Mycoplasma pulmonis (Mp) were generated. Each gene was sequenced in its entirety. The recA genes of Mm and Mp would encode proteins of 345 amino acids (aa) and 339 aa, respectively. The mycoplasmal RecA proteins revealed strong conservation when compared with RecA sequences from other bacterial species.

引用

页码：111 / 115

页数：5

共 20 条

[1] USE OF DEGENERATE PRIMERS FOR POLYMERASE CHAIN-REACTION CLONING AND SEQUENCING OF THE LACTOCOCCUS-LACTIS SUBSP LACTIS-RECA GENE [J].