VANADATE TREATMENT OF STREPTOZOTOCIN DIABETIC RATS RESTORES EXPRESSION OF THE INSULIN-RESPONSIVE GLUCOSE TRANSPORTER IN SKELETAL-MUSCLE

被引：82

作者：

STROUT, HV ^{[1
]}

VICARIO, PP ^{[1
]}

BISWAS, C ^{[1
]}

SAPERSTEIN, R ^{[1
]}

BRADY, EJ ^{[1
]}

PILCH, PF ^{[1
]}

BERGER, J ^{[1
]}

机构：

[1] BOSTON UNIV, SCH MED, DEPT BIOCHEM, BOSTON, MA 02118 USA

来源：

ENDOCRINOLOGY | 1990年 / 126卷 / 05期

关键词：

D O I：

10.1210/endo-126-5-2728

中图分类号：

R5 [内科学];

学科分类号：

1002 ; 100201 ;

摘要：

Streptozotocin-treated rats were diabetic, as assessed by blood glucose and plasma insulin values, while vanadate treatment restored blood glucose values to normal. Immu-noblot analysis using a monoclonal antibody to the insulin-responsive glucose transporter demonstrated a 70% decline in transporter expression in skeletal muscle of diabetic rats. Subsequent treatment of diabetic animals with vanadate resulted in renewed expression of the transporter to 87% of control levels. Northern blot analysis of total skeletal muscle RNA from diabetic animals revealed a 55% decline in the steady state level of muscle glucose transporter mRNA, while vanadate treatment led to a 187% increase in transporter mRNA over normal levels. These results support the conclusion that vanadate acts to relieve diabetic hyperglycemia by inducing expression of the insulin-responsive glucose transporter at the pretranslational level. © 1990 by The Endocrine Society.

引用

页码：2728 / 2732

页数：5

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