A LIGHT AND ELECTRON-MICROSCOPIC STUDY OF THE EFFECTS OF SURFACE-TOPOGRAPHY ON THE BEHAVIOR OF CELLS ATTACHED TO TITANIUM-COATED PERCUTANEOUS IMPLANTS

被引:91
作者
CHEHROUDI, B
GOULD, TRL
BRUNETTE, DM
机构
[1] UNIV BRITISH COLUMBIA,FAC DENT,DEPT ORAL BIOL,2199 WESBROOK MALL,VANCOUVER V6T 1Z7,BC,CANADA
[2] UNIV BRITISH COLUMBIA,FAC DENT,DEPT CLIN DENT SCI,VANCOUVER V6T 1Z7,BC,CANADA
来源
JOURNAL OF BIOMEDICAL MATERIALS RESEARCH | 1991年 / 25卷 / 03期
关键词
D O I
10.1002/jbm.820250310
中图分类号
R318 [生物医学工程];
学科分类号
0831 ;
摘要
Previous studies using light microscopy have demonstrated that micromachined grooved surfaces inhibit epithelial (E) downgrowth and affect cell orientation at the tissue/implant interface. This study investigates the ultrastructure of the epithelial and connective-tissue attachment to titanium-coated micromachined grooved, as well as smooth control, implant surfaces. V-shaped grooves, 3, 10, or 22-mu-m deep, were produced in silicon wafers by micromachining, replicated in epoxy resin, and coated with 50-nm titanium. These grooved, as well as smooth, titanium-coated surfaces were implanted percutaneously in the parietal area of rats and after 7 days processed for electron microscopy. The tissue preparation technique used in this study enabled us to obtain ultrathin sections with few artifacts from the area of epithelial and connective-tissue attachment. The histological observations demonstrated that E cells closely attached to, and interdigitated with, the 3-mu-m and 10-mu-m grooves. In contrast, E cells were not found inside the 22-mu-m-deep grooves and made contact only with the flat ridges between the grooves. As a general rule, fibroblasts (F) were oriented parallel to the long axis of the implants and produced a connective tissue capsule with 3-mu-m and 10-mu-m-deep grooved surfaces as well as smooth surfaces. On the 22-mu-m-deep grooved surfaces, however, F inserted obliquely into the implant. The attachment of F to the titanium surface was mediated by two zones; a thin (almost-equal-to 20 nm), amorphous, electron dense zone immediately contacting the titanium surface, and a fine fibrillar zone extending from the amorphous zone to the cell membrane. As oblique orientation of F has been associated with the inhibition of epithelial downgrowth, micromachined grooved surfaces of appropriate dimensions have the potential to improve the performance of percutaneous devices.
引用
收藏
页码:387 / 405
页数:19
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