A COMBINED PHYSICAL AND GENETIC-MAP OF PSEUDOMONAS-AERUGINOSA PAO

被引：72

作者：

RATNANINGSIH, E

DHARMSTHITI, S

KRISHNAPILLAI, V

MORGAN, A

SINCLAIR, M

HOLLOWAY, BW

机构：

[1] Department of Genetics, and Developmental Biology, Monash University, Clayton

来源：

JOURNAL OF GENERAL MICROBIOLOGY | 1990年 / 136卷

关键词：

D O I：

10.1099/00221287-136-12-2351

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

A combined physical and genetic map of Pseudomonas aeruginosa PAO was constructed by pulsed-field gel electrophoresis and Southern hybridization using cosmid clones from a genomic library carrying known genes. A total of 37 SpeI restriction fragments have been mapped on the 5862 kb genome, and fragment contiguity demonstrated by hybridization with clones from a SpeI junction fragment library and fragments obtained by partial SpeI digestion, both derived from the P. aeruginosa PAO chromosome.

引用

页码：2351 / 2357

页数：7

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