ENZYME MODIFICATION BY MPEG WITH AN AMINO-ACID OR PEPTIDE AS SPACER ARMS

被引：47

作者：

SARTORE, L ^{[1
]}

CALICETI, P ^{[1
]}

SCHIAVON, O ^{[1
]}

VERONESE, FM ^{[1
]}

机构：

[1] UNIV PADUA,DIPARTIMENTO SCI FARMACEUT,CNR,CTR CHIM FARMACO & PRODOTTI BIOLOGICAMENTE ATTIVI,I-35128 PADUA,ITALY

来源：

APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY | 1991年 / 27卷 / 01期

关键词：

SURFACE PROTEIN MODIFICATIONS; MONOMETHOXYPOLYETHYLENE GLYCOL; AMINO ACID OR PEPTIDE DERIVATIZED MPEG; ENZYMES FOR THERAPY; ARGINASE; RIBONUCLEASES AND SUPEROXIDE DISMUTASE;

D O I：

10.1007/BF02921514

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

A method for the modification of enzymes by MPEG carrying an amino acid or peptide as a spacer arm is described and tested with aliphatic or aromatic side chains amino acids. The procedure involves MPEG activation by p-nitrophenylchloroformate for the amino acid or peptide coupling that is in turn activated for the protein binding. The advantage of the method resides in the possibility to introduce proper reporter groups between the polymer and the protein as norleucine for a direct evaluation of the bound polymer chains, tryptophan for structural studies of the polymer-protein adduct, and radioactive amino acid for pharmacokinetic investigations. The method was positively tested with arginase, ribonuclease, and superoxide dismutase as enzymes of therapeutic value.

引用

页码：45 / 54

页数：10

共 16 条

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Abuchowsky A, 1981, ENZYMES DRUGS, P367

[2]

Brand L., 1967, METHOD ENZYMOL, V87, P776, DOI [10.1016/S0076-6879(67)11091-4, DOI 10.1016/S0076-6879(67)11091-4]

[3] PEG SUPEROXIDE-DISMUTASE DERIVATIVES - ANTIINFLAMMATORY ACTIVITY IN CARRAGEENAN PLEURISY IN RATS [J].