IDENTIFICATION OF 11-CIS AND ALL-TRANS-RETINAL IN THE PHOTORECEPTIVE ORGANELLE OF A FLAGELLATE GREEN-ALGA

被引：35

作者：

KREIMER, G ^{[1
]}

MARNER, FJ ^{[1
]}

BROHSONN, U ^{[1
]}

MELKONIAN, M ^{[1
]}

机构：

[1] UNIV COLOGNE,INST BIOCHEM,W-5000 COLOGNE 41,GERMANY

来源：

FEBS LETTERS | 1991年 / 293卷 / 1-2期

关键词：

RETINAL; PHOTOTAXIS; PHOTORECEPTIVE ORGANELLE; EYESPOT; GREEN ALGAE;

D O I：

10.1016/0014-5793(91)81150-7

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Isolation of intact photoreceptive organelles (eyespot apparatuses) involved in blue-light mediated photoresponses in a flagellate green alga (Spermatozopsis similis) allowed for the first time the identification of both 11-cis- and all-trans-retinal in a plant cell. Both isomers were identified by HPLC analysis in conjunction with UV spectra. Additionally, reconstitution of a distinct absorption band, centered around 540 nm, was achieved by addition of exogenous 9-cis-retinal to bleached, isolated eyespot apparatuses.

引用

页码：49 / 52

页数：4

共 25 条

[1]

BALOGHNAIR V, 1982, METHOD ENZYMOL, V88, P496

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