SOLID-PHASE ENZYME-LINKED-IMMUNOSORBENT-ASSAY FOR HEPATITIS-E VIRUS IGG AND IGM ANTIBODIES UTILIZING RECOMBINANT ANTIGENS AND SYNTHETIC PEPTIDES

被引：215

作者：

DAWSON, GJ ^{[1
]}

CHAU, KH ^{[1
]}

CABAL, CM ^{[1
]}

YARBOUGH, PO ^{[1
]}

REYES, GR ^{[1
]}

MUSHAHWAR, IK ^{[1
]}

机构：

[1] GENELABS INC,DEPT MOLEC VIROL,REDWOOD CITY,CA

来源：

JOURNAL OF VIROLOGICAL METHODS | 1992年 / 38卷 / 01期

关键词：

HEPATITIS-E VIRUS; HEPATITIS-E VIRUS RECOMBINANT ANTIGENS; HEPATITIS-E VIRUS SYNTHETIC PEPTIDES; HEPATITIS-E VIRUS IGG AND IGM ANTIBODIES;

D O I：

10.1016/0166-0934(92)90180-L

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Four recombinant antigens representing two distinct antigenic domains from two different strains of hepatitis E virus (HEV), were used individually to develop four ELISAs designed to detect antibodies to HEV. Both IgG and IgM class antibodies to HEV were detected in 7 of 8 pedigreed serum/plasma from known outbreaks of HEV in Mexico, Burma, Somalia and Pakistan. In addition, specific HEV-antibodies were detected in cynomolgus macaques following inoculation with various HEV strains. Anti-HEV was also detected in 8 of 386 (2.1%) randomly selected American blood donors. Supplemental tests utilizing both synthetic peptides and specific blocking assays provided additional serologic data confirming the presence of anti-HEV. Similar prevalence studies on a limited number of available sera from other geographical regions (Alaska, Japan, Germany, New Zealand, Thailand and Mexico) confirmed the presence of anti-HEV in at least 1.1 to 7.6% of the specimens.

引用

页码：175 / 186

页数：12

共 17 条

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BARANY G, 1980, PEPTIDES ANAL SYNTHE, V2, P1

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