EFFECTS OF OSMOTIC CULTURE CONDITIONS ON ISOLATED LICHEN MYCOBIONTS

Growth of the mycobiont of Evernia esorediosa under various osmotic culture conditions and its content of the dibenzofuran, hypostrepsilic acid, were examined. The growth rate and dibenzofuran content changed greatly when differing quantities of sucrose were added to the culture medium. Growth rate of the mycobiont was highest on the medium with 10% sucrose, while the content of hypostrepsilic acid was highest on medium containing 20% sucrose. The maximum growth of the mycobiont of Ramalina subbreviuscula was also on the medium with 10% sucrose.