IDENTIFICATION AND CHARACTERIZATION OF CHIROINOSITOL-CONTAINING PHOSPHOLIPIDS FROM BOVINE LIVER

被引：27

作者：

PAK, YB

LARNER, J

机构：

[1] Department of Pharmacology, University of Virginia, School of Medicine, Charlottesville

来源：

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 1992年 / 184卷 / 02期

关键词：

D O I：

10.1016/0006-291X(92)90696-I

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Glycosylphosphatidylinositols and phosphatidylinositols, postulated as precursor species of insulin mediators, were investigated for the presence of myoinositol, chiroinositol, glucosamine and galactosamine in their carbohydrate moities. Our study of bovine liver lipids shows heterogeneity in glycosylphosphatidylinositols with at least two species containing 96-97% chiroinositol and 95-100% galactosamine, with molar ratios of chiroinositol: phosphate: galactosamine: galactose: mannose (1:1.8:1:3:2 and 1:1.2:1:1.6:1). Another species contained predominantly myoinositol and glucosamine. Furthermore, we identified phosphatidylinositols with either myoinositol or chiroinositol present. Thus, distinct myoinositol and chiroinositol species of glycosylphosphatidylinositols and phosphatidylinositols are present in bovine liver. © 1992.

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页码：1042 / 1047

页数：6

相关论文

共 18 条

[1]

CHENG K, 1985, ANNU REV PHYSIOL, V45, P407

[2] IDENTIFICATION OF COVALENTLY BOUND INOSITOL IN THE HYDROPHOBIC MEMBRANE-ANCHORING DOMAIN OF TORPEDO ACETYLCHOLINESTERASE [J].

FUTERMAN, AH ;

LOW, MG ;

ACKERMANN, KE ;

SHERMAN, WR ;

SILMAN, I .

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 1985, 129 (01) :312-317

[3] GLUCOSE-TRANSPORT AND ANTILIPOLYSIS ARE DIFFERENTIALLY REGULATED BY THE POLAR HEAD GROUP OF AN INSULIN-SENSITIVE GLYCOPHOSPHOLIPID [J].

KELLY, KL ;

MATO, JM ;

MERIDA, I ;

JARETT, L .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1987, 84 (18) :6404-6407

[4]

LARNER J, 1989, ANN NY ACAD SCI, V573, P297, DOI 10.1111/j.1749-6632.1989.tb15006.x

[5] INSULIN MEDIATORS - STRUCTURE AND FORMATION [J].

LARNER, J ;

HUANG, LC ;

TANG, G ;

SUZUKI, S ;

SCHWARTZ, CFW ;

ROMERO, G ;

ROULIDIS, Z ;

ZELLER, K ;

SHEN, TY ;

OSWALD, AS ;

LUTTRELL, L .

COLD SPRING HARBOR SYMPOSIA ON QUANTITATIVE BIOLOGY, 1988, 53 :965-971

[6] RAT-LIVER INSULIN MEDIATOR WHICH STIMULATES PYRUVATE-DEHYDROGENASE PHOSPHATASE CONTAINS GALACTOSAMINE AND D-CHIROINOSITOL [J].

LARNER, J ;

HUANG, LC ;

SCHWARTZ, CFW ;

OSWALD, AS ;

SHEN, TY ;

KINTER, M ;

TANG, G ;

ZELLER, K .

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 1988, 151 (03) :1416-1426

[7]

LOW MG, 1987, BIOCHEM J, V244, P1

[8] REMOVAL OF COVALENTLY BOUND INOSITOL FROM TORPEDO ACETYLCHOLINESTERASE AND MAMMALIAN ALKALINE-PHOSPHATASES BY DEAMINATION WITH NITROUS-ACID - EVIDENCE FOR A COMMON MEMBRANE-ANCHORING STRUCTURE [J].

LOW, MG ;

FUTERMAN, AH ;

ACKERMANN, KE ;

SHERMAN, WR ;

SILMAN, I .

BIOCHEMICAL JOURNAL, 1987, 241 (02) :615-619

[9] A PUTATIVE MEDIATOR OF INSULIN ACTION WHICH INHIBITS ADENYLATE-CYCLASE AND ADENOSINE 3',5'-MONOPHOSPHATE-DEPENDENT PROTEIN-KINASE - PARTIAL-PURIFICATION FROM RAT-LIVER - SITE AND KINETIC MECHANISM OF ACTION [J].

MALCHOFF, CD ;

HUANG, L ;

GILLESPIE, N ;

PALASI, CV ;

SCHWARTZ, CFW ;

CHENG, K ;

HEWLETT, EL ;

LARNER, J .

ENDOCRINOLOGY, 1987, 120 (04) :1327-1337

[10] PARTIAL STRUCTURE OF AN INSULIN-SENSITIVE GLYCOPHOSPHOLIPID [J].

MATO, JM ;

KELLY, KL ;

ABLER, A ;

JARETT, L ;

CORKEY, BE ;

CASHEL, JA ;

ZOPF, D .

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 1987, 146 (02) :764-770