SYNTHESIS AND RIBOSOME BINDING-PROPERTIES OF MODEL MESSENGER-RNAS MODIFIED WITH UNDECAGOLD CLUSTER

被引：10

作者：

SKRIPKIN, E

YUSUPOVA, G

YUSUPOV, M

KESSLER, P

EHRESMANN, C

EHRESMANN, B

机构：

[1] RUSSIAN ACAD SCI, INST PROTEIN RES, PUSHCHINO 142292, RUSSIA

[2] CEA, CTR ETUDES SACLAY, DIEP, F-91191 GIF SUR YVETTE, FRANCE

来源：

BIOCONJUGATE CHEMISTRY | 1993年 / 4卷 / 06期

关键词：

D O I：

10.1021/bc00024a019

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

The synthesis and purification of short model messenger RNAs modified with undecagold cluster are described. A monoamino undecagold cluster was introduced on the oxidized 3' cis-glycol group of the mRNA followed by reduction of the formed Schiff s base. The stability of the modified mRNA under the conditions used for in vitro messenger RNA translation is studied. The possibility of the formation of a specific translational initiation complex with bacterial ribosomes and modified mRNAs is shown. The results of these experiments indicate that the attachment of an undecagold cluster to a mRNA is a useful tool for electron microscopic and crystallographic studies.

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页码：549 / 553

页数：5

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