PRODUCTION OF CEPHALOSPORIN-C BY IMMOBILIZED CEPHALOSPORIUM-ACREMONIUM IN POLYETHYLENEIMINE-MODIFIED BARIUM ALGINATE

被引：21

作者：

PARK, HJ ^{[1
]}

KHANG, YH ^{[1
]}

机构：

[1] YEUNGNAM UNIV,DEPT APPL MICROBIOL,KYONGSAN 712749,SOUTH KOREA

来源：

ENZYME AND MICROBIAL TECHNOLOGY | 1995年 / 17卷 / 05期

关键词：

CEPHALOSPORIUM ACREMONIUM; CEPHALOSPORIN C; POLYETHYLENEIMINE; BARIUM ALGINATE; CALCIUM ALGINATE; STRONTIUM ALGINATE; FED-BATCH FERMENTATION; CELL IMMOBILIZATION;

D O I：

10.1016/0141-0229(94)00076-4

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

Calcium, barium, and strontium alginates were studied to develop a suitable gel matrix for the immobilization of Cephalosporium acremonium. Barium alginate gave better results in both gel stability and cell activity than Ca- and Sr-alginates. The mechanical stability of Ba-alginate was greatly enhanced by the use of polyethyleneimine (PEI). According to quantitative studies of two factors, PEI concentration and Ba-alginate bead size, cephalosporin C (CPC) production was predominately influenced by the bead size alone. Thus, C. acremonium cells were immobilized in microbeads of PEI-modified Ba-alginate. Minimal production media, which increased CPC productivity 3.4-fold compared with chemically defined media, were used in fed-batch fermentations. Cephalosporin C was produced continuously for 30 days and CPC yields of immobilized cells in fed-batch fermentation increased about sevenfold as compared with those of free cells in batch fermentation. Immobilized cells in PEI-modified Ba-alginate were reusable for more than 30 days.

引用

页码：408 / 412

页数：5

共 21 条

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