FLOW-INJECTION ANALYSIS FOR L-GLUTAMATE USING IMMOBILIZED L-GLUTAMATE OXIDASE - COMPARISON OF AN ENZYME REACTOR AND ENZYME ELECTRODE

被引：51

作者：

YAO, T

KOBAYASHI, N

WASA, T

机构：

[1] Department of Applied Chemistry, College of Engineering, University of Osaka Prefecture, Sakai, 591, Mozu-Umemachi

来源：

ANALYTICA CHIMICA ACTA | 1990年 / 231卷 / 01期

关键词：

D O I：

10.1016/S0003-2670(00)86405-3

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

An enzyme electrode and enzyme based on immobilized l-glutamate oxidase are used for the determination of l-glutamate in a flow-injection system. The hydrogen peroxide produced is monitored amperometrically. The enzyme reactor system surpasses the enzyme electrode system with regard to sensitivity and analytical speed. For both systems, the peak current is linearly related to the l-glutamate concentration in the range 5 × 10-6-1 × 10-3 M. l-Glutamate in seasoning can be determined very selectively with < 0.7% r.s.d. © 1990.

引用

页码：121 / 124

页数：4

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